Bone morphogenetic protein-15 (BMP-15) is a member of the TGF-beta superfamily known to regulate ovarian functions in mammals. Recently, we cloned zebrafish BMP-15 (zfBMP-15) cDNA and demonstrated that it may play a role in oocyte maturation. In this study, we further investigated the role of BMP-15 in zebrafish follicular development and oocyte maturation using an antiserum developed for zfBMP...

objective: nutrients and antioxidants in the medium of immature oocyte have a profound effect on maturation, fertilization and development of resulting embryos. in this study the effects of melatonin as an antioxidant agent on maturation, glutathione level and expression of high mobility group box-1 (hmgb1) gene were evaluated in immature oocytes of mice stained with brilliant cresyl blue (bcb)...

Normal oocyte maturation depends on signal transmission between granulosa cells and the oocyte. We have analysed the effects of inhibiting (I) cyclic AMP-dependent protein kinase (protein kinase A, PK-A), (II) Ca2+/phospholipid-dependent protein kinase (protein kinase C, PK-C) and (III) calmodulin (CaM) on pig oocyte maturation in vitro, protein synthesis and phosphorylation. The inhibition of ...

Accurate estimates of reproductive parameters important in understanding life history evolution and conservation small fishes are dependent upon careful assignment ovarian phases. Proper is based the stages propagule (oocyte) development, oocyte maturation location any clutch or portion thereof within ovaries. Macroscopic inspection developmental phases have often been used for freshwater fishe...

Background: In vitro maturation is a good method to decrease cancer risk of superovulation by gonadotropin hormones. A paracrine effect of oocyte secretions on oocyte developmental competence is under investigation. Apart from oocyte maturation, ovulation in vivo requires a precise control of extracellular matrix modification. Cumulus cells secrete hyaluronan to form a muco-elastic extracellula...

sea cucumbers saponins are triterpenoid glycosides which exert beneficial biomedical effects. this study was performed to assess the effect of saponin extracted from sea cucumber holothuria leucospilota            ( h. leucospilota ) on maturation of mice oocytes and granulosa cells. the germinal vesicles oocytes were collected from 6–8 weeks old naval medical research institute (nmri) mice ova...

BACKGROUND In a growth phase occurring during most of folliculogenesis, the oocyte produces and accumulates molecules and organelles that are fundamental for the development of the preimplantation embryo. At ovulation, growth is followed by a phase of maturation that, although confined within a short temporal window, encompasses modifications of the oocyte chromosome complement and rearrangemen...

For human IVF, the patient's ovaries are hormonally stimulated to ensure the collection of fully matured oocytes that are at the metaphase II stage. Only these oocytes can be successfully fertilized either when mixed with sperm or after ICSI. Nevertheless, in some cases immature or maturing oocytes are recovered from follicles. Surprisingly, sometimes these oocytes do not complete maturation wh...

Oocytes of Pleurodeles waltl were activated after in vivo maturation by needle pricking or electric shock. After in vitro maturation, the oocytes were not activated by these stimuli. Coelomic oocytes and the oocytes which began their maturation in vivo could be activated by electric shock. During in vivo oocyte maturation, the activity of glucose-6-phosphate dehydrogenase (G6PDH), the key enzym...