H2O2-induced cytotoxicity in normal human pulmonary fibroblasts (HPFs) is of interest in toxicological research since HPFs are involved in lung inflammation, fibrosis and cancer. The present study investigated the cytotoxic effects of H2O2 on normal HPFs in relation to reactive oxygen species (ROS) and mitogen-activated protein kinases (MAPKs) using the well-known antioxidants N-acetyl cysteine...

The relationship of intracellular pH (pHi) to superoxide radical (O2-) generation was investigated in chemotactic factor-stimulated human neutrophils. Exposure of cells to 100 nM N-formylmethionyl-leucyl-phenylalanine (FMLP) caused activation of Na/H exchange which, in 140 mM Na medium (pH0 7.40), led to a rise in pHi from 7.22 to 7.80. This pHi change was sensitive to amiloride (apparent Ki 78...

In this issue of the Journal of Applied Physiology, Mizuno and colleagues (5) report the use of positron emission tomography (PET) in human skeletal muscle (knee extensors) both at rest and at 30 min after exhaustive single-leg cycling. They were able to measure regional perfusion and O2 consumption in five areas situated at an equal distance over a proximal-todistal axis of 12 cm. Their princi...

BACKGROUND AND PURPOSE Experimental evidence supports a major role of increased intracellular calcium [Ca2+]i levels in the induction of neuronal damage during cerebral ischemia. However, the source of Ca2+ rise has not been fully elucidated. To clarify further the role and the origin of Ca2+ in cerebral ischemia, we have studied the effects of various pharmacological agents in an in vitro mode...

Background and Purpose—Experimental evidence supports a major role of increased intracellular calcium [Ca]i levels in the induction of neuronal damage during cerebral ischemia. However, the source of Ca rise has not been fully elucidated. To clarify further the role and the origin of Ca in cerebral ischemia, we have studied the effects of various pharmacological agents in an in vitro model of o...

We tested the hypothesis that vascular macrophage infiltration and O2 (-) release impairs sympathetic nerve α2-adrenergic autoreceptor (α2AR) function in mesenteric arteries (MAs) of DOCA-salt hypertensive rats. Male rats were uninephrectomized or sham operated (sham). DOCA pellets were implanted subcutaneously in uninephrectomized rats who were provided high-salt drinking water or high-salt wa...

In this study, the malondialdehyde (MDA) levels in the muscle, liver and kidney tissues and the antioxidant vitamin (A, C, E) levels in the muscle tissue of juvenile rainbow trout were investigated under different oxygen levels (3.5 mg O2/l, 4.5 mg O2/l and 7 mg O2/l) at the end of the 8 hour trial. Fish (initial weight and length, 30.19±2.2 g and 13.15±0.22 cm, respectively) were distributed i...

In this study, the malondialdehyde (MDA) levels in the muscle, liver and kidney tissues and the antioxidant vitamin (A, C, E) levels in the muscle tissue of juvenile rainbow trout were investigated under different oxygen levels (3.5 mg O2/l, 4.5 mg O2/l and 7 mg O2/l) at the end of the 8 hour trial. Fish (initial weight and length, 30.19±2.2 g and 13.15±0.22 cm, respectively) were dis...