Plasma clearance of D-sorbitol, a nontoxic polyol, occurs predominantly in the liver and has been used to measure functional liver blood flow after bolus and steady- state intravenous administration. However, it is not known which of these two administration methods is superior. Therefore, plasma D-sorbitol clearance was studied in an animal model both after a bolus dose and under steady-state ...

A survey of the microbial quality based on enterobacteriaceae counts of the table eggs sold in Egyptian markets was conducted to evaluate their quality and the possibility of presence of Enterovirulent Escherichia coli strains. Six hundred random table egg samples were collected from different shops, supermarkets and homes. Each six pooled eggs constituted a composite sample. Higher enterobacte...

INTRODUCTION Fresh produce has been implicated in a number of documented outbreaks of foodborne illness caused by bacteria, viruses, and parasites. Shiga toxin-producing Escherichia coli (STEC) have been detected on vegetables, raising concerns about the prevalence of E. coli contamination in produce, which can take place at various points from farm to fork. This study aimed to detect the prese...

BACKGROUND Sepsis-induced microcirculatory alterations contribute to tissue hypoxia and organ dysfunction. In addition to its plasma volume expanding activity, human serum albumin (HSA) has anti-oxidant and anti-inflammatory properties and may have a protective role in the microcirculation during sepsis. The concentration of HSA infused may influence these effects. We compared the microcirculat...

Antiserum raised against intimin from enterohemorrhagic Escherichia coli (EHEC) O157:H7 strain 86-24 has been shown previously by our laboratory to inhibit adherence of this strain to HEp-2 cells. In the present study, we sought to identify the region(s) of intimin important for the effect of anti-intimin antisera on EHEC adherence and to determine whether antisera raised against intimin from a...

Sepsis is frequently the cause of severe pulmonary dysfunction. Gentamicin is used clinically due to its wide spectrum of activities against infections; however it has serious adverse effects and life-threatening toxicity. The challenge for scientists is to develop alternative therapy or protective agents against its toxicity. Hence the present study is an attempt to elucidate the possible prot...

Enteropathogenic Escherichia coli isolated from diarrheal children and adults were studied. Twenty-two OK groups were set up as enteropathogenic Eschericha to include : O26: K60, O28a, 28c : K73, O44: K74, O55: K59, O86: K61, O86: K62, O111: K58, O112a,112c : K66, 0119: K69, O124: K72, O125: K70, O126: K71, O127: K61, O86: K63, O128: K67, O136: K78, O143: Kx1, O144: Kx2, O146: K87, A-D (Alkales...

Attaching and effacing Escherichia coli (AEEC) strains cause histopathological alterations termed “attaching and effacing (A/E) lesions” (8). The ability to cause A/E lesions is encoded on a large bacterial chromosomal pathogenicity island, the locus of enterocyte effacement (LEE). The central portion of LEE encodes intimin (Eae, 94to 97-kDa outer membrane protein) and Tir, the intimin receptor...

•Structure of native EspA adopts a unique fold compared with other T3SS tip proteins•Native different conformation from the chaperone-bound state•The structure reveals an electropositive pattern within its lumen•In vivo studies show that electrostatics play crucial role in function The type III secretion system (T3SS) is virulence mechanism employed by Gram-negative pathogens. forms proteinaceo...