We have developed a system for analyzing recombination between a DNA fragment released in the nucleus from a single-copy plasmid and a genomic target in order to determine the influence of DNA sequence mismatches on the frequency of gene replacement in Saccharomyces cerevisiae. Mismatching was shown to be a potent barrier to efficient gene replacement, but its effect was considerably ameliorate...

The protein talin has two domains of approximately 200 and 47 kD, which can be cleaved apart by a variety of proteases. To examine the function of these two structural domains of talin, we have digested purified talin with a calcium-dependent protease and separated the resulting fragments chromatographically. Both fragments were radioiodinated and used to probe Western blots of whole fibroblast...

introduction: the antinociceptive effect of morphine is, in part, mediated through the activation of a descending pathway. one of the major components of this pathway is the nucleus raphe magnus (nrm). our previous study demonstrated the involvement of nrm in the analgesic effect of morphine microinjected into the nucleus cuneiformis (ncf) in a descending manner. the aim of the current study wa...

a b s t r a c tintroduction: the nucleus cuneiformis (ncf) and ventrolateral periaqueductal gray (vlpag), two adjacent areas, mediate the central pain modulation and project to the nucleus raphe magnus (nrm). methods: this study examined whether the antinociceptive effect of morphine microinjected into the ncf is influenced by inactivation of vlpag and nrm in rats. animals were bilaterally micr...

The $^{24}\mathrm{Mg}+^{12}\mathrm{C}$ fusion reaction was used to perform a detailed $\ensuremath{\gamma}$-ray spectroscopy study of the astrophysically important nucleus $^{34}\mathrm{Ar}$. In particular, an experimental setup, coupling advanced tracking array GRETINA with well-established Argonne fragment mass analyzer (FMA), employed obtain excitation energies and spin-parity assignments fo...

The dynamic nature of the nuclear envelope (NE) is often underestimated. NE protects, regulates, and organizes eukaryote genome adapts to epigenetic changes its environment. morphology characterized by a wide range diversity abnormality such as invagination blebbing, it diagnostic factor for pathologies cancer. Recently, micronuclei, small nucleus that contains full chromosome or fragment there...

In response to extracellular stimuli, mitogen-activated protein kinase (MAPK, also known as ERK), which localizes to the cytoplasm in quiescent cells, translocates to the nucleus and then relocalizes to the cytoplasm again. The relocalization of nuclear MAPK to the cytoplasm was not inhibited by cycloheximide, confirming that the relocalization is achieved by nuclear export, but not synthesis, ...

U2 small nuclear ribonucleoprotein (snRNP) auxiliary factor 65 kDa (U2AF65) is an essential splicing factor in the recognition of the pre-mRNA 3' splice sites during the assembly of the splicing commitment complex. We report here that U2AF65 is proteolyzed during apoptosis. This cleavage is group I or III caspase dependent in a noncanonical single site localized around the aspartic acid(128) re...

The distortion on the intermittency signal, due to detection efficiency and to the presence of pre–equilibrium emitted particles, is studied in a schematic model of nuclear multi-fragmentation. The source of the intermittency signal is modeled with a percolating system. The efficiency is schematized by a simple function of the fragment size, and the presence of pre–equilibrium particles is simu...