Cyanocycline A was cytotoxic against Meth A cells in vitro, and also showed marked activity against the same cell line grown as an experimental ascites tumor. This antibiotic inhibited nucleic acid synthesis in Escherichia coli and Meth A cells. The antimicrobial activity of the antibiotic was reversed by addition of exogenous herring sperm DNA. Cytofluorometric analysis of cyanocycline A-treat...

Nucleic acid molecules are important therapeutic agents in the field of antisense oligonucleotide, RNA interference, and gene therapies. Since nucleic acids are not able to cross cell membranes and enter efficiently into cells on their own, the development of efficient, safe, and precise delivery systems is the crucial challenge for development of nucleic acid therapeutics. For the delivery of ...

We describe the synthesis of a family of arylamine-substituted papers which can be diazotized and coupled with nucleic acids. The synthesis is simple and uses readily obtainable starting materials. A partial characterization of the nucleic acid binding activity is reported, as well as a demonstration of the utility of the activated paper for the detection of electrophoretically separated RNA by...

PERSPECTIVES AND UMMARY .............................................................. 1152 INTRODUCTION ..................................................................................... 1155 ORGANIC AND STRUCTURAL CHEMISTRY OF PSORALEN AND ITS ADDUCTS ................................................................... 1158 Naturally Occurring Psoralens ........................................

Certain purines, notably adenine, are readily incorporated into the nucleic acids of rat tissues (1). Studies with glycine, a specific precursor involved in the synthesis of purines, in the same animal have shown (2, 3) relative uptakes in the ribose nucleic acid (RNA) and deoxyribose nucleic acid (DNA) fractions which are somewhat different from those obtained with adenine (4). A simultaneous ...

the drug occurred with the formation of one or more substances which was inhibitory to each of the 3 systems. The inhibitory action was approximately equally manifest against DNA and RNA synthesis. The inhibition of protein synthesis was delayed in onset and was likely a consequence of a diminished rate of nucleic acid synthesis. Evidence was obtained which indicated that the inhibitory compone...

Addition of 1 @Mmethotrexate to cultures of L5178Y cells results in an initial inhibition ofthymidine, uridine, and leucine incorporation into acid-insoluble material followed, after about 10 hr. by a partial recovery in the extent of incorporation of these precursors. Acid-soluble adenosine triphosphate and guanosine triphosphate concentrations are greatly reduced initially, but guanosine trip...

In previous papers of this series, experimental conditions have been described which will enable the following processes to be studied in washed suspensions of Staphylococcus aureus (Micrococcus pyogenes var. aureus): internal accumulation of free glutamic acid (Gale, 1947 a, b), extracellular accumulation of peptides containing glutamic acid (Gale & Van Halteren, 1951), accumulation of combine...

The biological activity of 6mercaptopurine, an analogue of hypoxanthme, has been the subject of extensive studies, but the mechanism of its action has remained unclear. This drug inhibits the growth of bacteria (l-3) and mammalian cells (4, 5) in vitro and the proliferation of experimental tumors and leukemias in tiuo (6-9). It has been suggested that it interferes with the synthesis of nucleic...

1. Mycophenolic acid, an antibiotic of some antiquity that more recently has been found to have marked activity against a range of tumours in mice and rats, strongly inhibits DNA synthesis in the L strain of fibroblasts in vitro. 2. The extent of the inhibition of DNA synthesis is markedly increased by preincubation of the cells with mycophenolic acid before the addition of [(14)C]thymidine. 3....