objective: cd44+/cd24-/low breast cancer cells have tumour-initiating properties with stem cell-like features. breast cancer gene 1 (brca1) is a tumour suppressor gene that plays a crucial role in dna repair and maintenance of chromosome stability. the clinicopathological features of breast cancer in brca1 mutation carriers suggest that brca1 may function as a stem-cell regulator. materials and...

Introduction: We have developed a diagnostic marker hyperpolarized 1,4-C2-fumarate, which takes advantage of the technique dynamic nuclear polarization for magnetic resonance imaging (DNP-MRI). This marker allows real-time metabolic studies of a TCAcycle intermediate, C-malate. With this marker we have investigated the metabolism in the liver of CCl4 treated rats. Intra gastric administration o...

BACKGROUND Automated scanning devices and image analysis software provide a means to overcome the limitations of manual semiquantitative scoring of immunohistochemistry. Common drawbacks to automated imaging systems include an inability to classify tissue type and an inability to segregate cytoplasmic and nuclear staining. METHODS Immunohistochemistry for the membranous marker α-catenin, the ...

We investigate the ground state of a free massless (pseudo)scalar field in 1+1– dimensional space–time. We argue that in the quantum field theory of a free massless (pseudo)scalar field without infrared divergences (Eur. Phys. J. C 24, 653 (2002)) the ground state can be represented by a tensor product of wave functions of the fiducial vacuum and of the collective zero–mode, describing the moti...

We investigate the ground state of a free massless (pseudo)scalar field in 1+1– dimensional space–time. We argue that in the quantum field theory of a free massless (pseudo)scalar field without infrared divergences (Eur. Phys. J. C 24, 653 (2002)) the ground state can be represented by a tensor product of wave functions of the fiducial vacuum and of the collective zero–mode, describing the moti...

Six DNA regions were evaluated as potential DNA barcodes for Fungi, the second largest kingdom of eukaryotic life, by a multinational, multilaboratory consortium. The region of the mitochondrial cytochrome c oxidase subunit 1 used as the animal barcode was excluded as a potential marker, because it is difficult to amplify in fungi, often includes large introns, and can be insufficiently variabl...

Nuclear texture analysis measures the spatial arrangement of the pixel gray levels in a digitized microscopic nuclear image and is a promising quantitative tool for prognosis of cancer. The aim of this study was to evaluate the prognostic value of entropy-based adaptive nuclear texture features in a total population of 354 uterine sarcomas. Isolated nuclei (monolayers) were prepared from 50 µm ...

Autoimmune myositis encompasses various myositis-overlap syndromes, each being identified by the presence of serum marker autoantibodies. We describe a novel myositis-overlap syndrome in 4 patients characterized by the presence of a unique immunologic marker, autoantibodies to nuclear pore complexes. The clinical phenotype was characterized by prominent myositis in association with erosive, ant...