Chromosomal abnormalities represent a significant genetic cause of male infertility because they impair spermatogenesis. The objective the current study was to determine prevalence and distribution chromosomal in Greek men with infertility. Four hundred eighty-eight infertile (27 azoospermic, 168 oligospermia -98 mild, 57 moderate, 13 severe- 293 normospermia) undergoing In <em>Vitro <...

background: glycosidases profusion in male reproductive fluids suggests a possible relationship with sperm function. although hexosaminidase (hex) is the most active glycosidase in epididymal fluid and seminal plasma, as well as in spermatozoa, glucosidase is considered a marker for epididymal function and azoospermia. objective: the aim of this study was to determine hex activity in seminal pl...

We established a quick and reliable method for recovering cell-free seminal DNA (cfsDNA), by using the binding-washing-elution procedure on the DNA purification column. Low variations (below 15%) among the triplicate values of cfsDNA quantity verified the reproducibility of our cfsDNA recovery method. Similar cfsDNA yield and size distribution between seminal plasma acquired by filtration and c...

BACKGROUND In view of the high cure rates in patients with testicular germ cell tumors (TGCT), increasing clinical importance is attached to protection of fertility. Long-term infertility due to cytostatic therapy may be expected in more than 50% of the patients at a cumulative dose of cisplatin > 0.6 g/m2. The standard procedure for fertility protection in cancer patients includes cryopreserva...

Background: Stimulatory therapy with gonadotrpins is an effective treatment to induce spermatogenesis in men with idiopathic hypogonadotroptic hypogonadism (IHH). The aim of this study was to assess the effectiveness of human chorionic gonadotropin / human menopausal gonadotropin on hypogonadotropic infertile men. Materials and Methods: This study included fifty-six azoospermic infertile men wi...

BACKGROUND Testicular sperm retrieval can lead to paternity for azoospermic patients with spermatogenic failure. The human BOULE gene, a meiotic regulator of germ cells, is a gene whose altered expression may be associated with sterility. We determined the levels of BOULE transcripts in the testes of azoospermic patients, and evaluated the relationship between BOULE transcript levels and patien...

Recent studies have shown Y chromosome microdeletions associated with male infertility. The factors responsible for Y chromosome microdeletions in spermatozoa remain unresolved. However, the environmental pollutants are known to damage DNA in differentiating and maturing germ cells in the male reproductive tract. Therefore, the aim of this study was to investigate the effects of seminal hexachl...

BACKGROUND The aim of this study was to assess the use of testicular sperm for ICSI in azoospermic men with hypogonadotrophic hypogonadism unresponsive to gonadotrophin therapy. METHODS Fifteen patients with hypogonadotrophic hypogonadism who remained azoospermic after hormonal treatment underwent testicular sperm extraction (TESE) and ICSI. These men were recruited from the Egyptian IVF cent...

OBJECTIVE To measure the zinc level in fertile and infertile population and to find out relationship of serum and seminal plasma zinc levels and serum testosterone in oligospermic and azoospermic infertile men. DESIGN Comparative study. PLACE AND DURATION OF STUDY The Infertility Clinic, Jinnah Postgraduate Medical Center (JPMC), Karachi, from January 2002-July 2003. PATIENTS AND METHODS ...