Hairy root induction in plants has affected by many factors including type, age of explant and bacterial strain. In the present study, the effects of bacterial strains (A4, C58, 15834 and GM), two concentrations (OD600= 0.5 and 1) and two explants (leaves and stems) in hairy root induction of C. blumei</...

BACKGROUND Idiopathic pulmonary fibrosis (IPF) is a fatal lung disease with no known effective therapy. It is often assumed, but has not been objectively evaluated, that pulmonary inflammation subsides as IPF progresses. The goal of this work was to assess changes in the degree of inflammatory cell infiltration, particularly lymphocytic infiltration, over the duration of illness in IPF. METHO...

PURPOSE To describe the phenotypic characteristics of a limbal epithelial cell sheet outgrowth from a limbal explant cultured on amniotic membrane. METHOD Immunofluorescent staining and confocal microscopy were used to examine the expressions of p63, Ki-67, keratins 3 and 14, connexin 43, and the integrin alpha6/beta4 and alpha3/beta1 subunits in corneal and limbal tissues in a limbal explant...

The medium pH level of plant tissue cultures has been shown to be essential to many aspects of explant development and growth. Sensitivity or tolerance of medium pH change varies according to specific requirements of in vitro individual species. The objectives of this study are to 1) determine medium pH change over time in storage conditions and with presence of explants, 2) evaluate the effect...

Cultured embryonic and adult skeletal muscle cells have a number of different uses. The micro-dissected explants technique described in this chapter is a robust and reliable method for isolating relatively large numbers of proliferative skeletal muscle cells from juvenile, adult or embryonic muscles as a source of skeletal muscle stem cells. The authors have used micro-dissected explant culture...

background: in this study we evaluated the prognostic impact of metastatic lymph node ratio (mlr) in patients with gastric cancer undergoing curative surgical resection. methods: a total of 121 patients with gastric adenocarcinoma undergoing curative r0 surgical resection in our institutions between 2003 and 2010 were included in this study.  mlr was classified into 3 groups as follows: mlr0; 0...

Several studies have shown that decidual tissue produces soluble factors which can mediate a variety of immunosuppressive activities. In order to assess whether prostaglandin E2 (PGE2) was present in supernatants produced by explant culture of 10 human specimens of first trimester decidua. The effect of the supernatants from the two different series (with and without indomethacin treatment) ...

Stevia rebaudiana is a herbaceous perennial plant with great global demand due to its beneficial steviol glycosides(SGs) content. Current conventional breeding technique unable cater the need for more S. planting materials. Therefore, an improved in vitro shoot regeneration protocol was developed by using chitosan. The highest fresh weight of (0.586 ± 0.176 g/explant), dry (0.056 0.02 g/explant...

Silicon, which is widely used in different fields, has been plant production vivo and vitro studies recent years. Especially studies, it seen that its effect on growth development examined. In this study, the of three silicon sources their four concentrations micro-propagation 140 Ru grape rootstocks was investigated. as explants one-node micro cuttings rootstock MS (Murashige Skoog) nutrient m...