Microrobots hold promise for applications ranging from targeted delivery to enhanced mixing at the microscale. However, current fabrication techniques suffer limited throughput and material selection. Here, we demonstrate a versatile route enabling synthesis of microrobots off-the-shelf micro- nano-particles. Our protocol hinges on toposelective attachment photocatalytic nanoparticles onto micr...

The human sulfatase family has 17 members, 13 of which have been characterized biochemically. These enzymes specifically hydrolyze sulfate esters in glycosaminoglycans, sulfolipids, or steroid sulfates, thereby playing key roles in cellular degradation, cell signaling, and hormone regulation. The loss of sulfatase activity has been linked to severe pathophysiological conditions such as lysosoma...

The activities of arylsulfatases A and B were determined in human primary and secondary tumor tissues (total, 53 cases) of various histological types. Significantly higher activities of these sulfatases were found in almost all the primary lung carcinomas as compared to their corresponding uninvolved tissues. No significant correlation was demonstrated between the enzyme activities and histolog...

The kinetics of the photocatalytic degradation of 4-nitrophenol (4-NP) in the presence of TiO2 has been investigated experimentally and theoretically. The effects of the catalyst loading, the initial concentration of 4-NP, H2O2 and the added Cu2+ ions on the degradation rate have been examined. A pseudo-first order kinetic model has been used to describe the results. A linear dependence of the ...

In this paper, we reported the construction of a hybrid biosensor for direct, highly selective, sensitive, and rapid quantitative determination of organophosphate pesticides with p-nitrophenyl substituent using purified organophosphorus hydrolase (OPH) for the initial hydrolysis and Arthrobacter sp. JS443 for subsequent p-nitrophenol oxidation. The biocatalytic layer was prepared by co-immobili...

In slightly acidic medium V(IV) forms with 4-nitrocatechol (NC) and 3-(4,5dimethyl-2-thiazol)-2,5-diphenyltetrazolium bromide (Thiazolyl Blue Tetrazolium, MTT) a well extractable into chloroform, intensively colored (ε405=3.5×10 l mol cm) 1:2:2 complex. The optimal conditions for extractive-spectrophotometric determination of V(IV) were found: pH 4.0-4.8, CNC= 2.9×10 mol l, CMTT= 1.6×10 4 mol l...

Sulphatase preparations from Abalone entrails, the limpet Patella vulgata and ox liver, as well as artificial substrates for these enzymes, were used in the hamster in vitro fertilization system to study the possible roles of sperm sulphatases in sperm-zona pellucida interactions. p-nitrophenyl sulphate, p-nitrocatechol sulphate, ascorbate 2-sulphate, as well as D-glucopyranose and D-galactopyr...

A method is described for the preparation of a B-galactosidase which acts on the brain lipid, ceramide galactoside. The enzyme is obtained from particles that sediment between I 000-13 ooo xg from rat brains and is extracted with a 60,& sodium cholate solution. The enzyme is solubilized and inactive proteins removed by digestion with pancreatic enzymes and the cholate is removed by dialysis and...

The Aerodyne aerosol mass spectrometer (AMS) is used extensively to measure the chemical composition of non-refractory submicron particles for laboratory and atmospheric field studies. Typical AMS calibration methods are mass-based, involving generating pure or mixtures with a known size number concentration. Here we present new method using heated platinum/molybdenum catalyst-based conversion ...