AIM To investigate the effect of vitamin C and E supplementation in the levels of nitrite, nitric oxide (NO) related metabolite, and ocular surface parameters in diabetic patients. METHODS 50 patients with non-insulin dependent diabetes mellitus were given vitamin C (1000 mg/day) and vitamin E (400 IU/day) supplementation for 10 days. Nitrite levels in tears were measured by photometric deter...

background: the assessment of altered nitric oxide (no) availability is of potentially important diagnostic and prognostic significance. the present study is aimed to investigate the effect of l-arginine (as a natural no donor) supplementation on no metabolite in a rabbit model of hypercholesterolemia to find a reliable marker for endothelial no production. methods: white male rabbits (n = 30) ...

It is well-established that plant hemoglobins (Hbs) are involved in nitric oxide (NO) metabolism via NO dioxygenase and/or nitrite reductase activity. The ferrous-deoxy Arabidopsis Hb1 and Hb2 (AHb1 and AHb2) have been shown to reduce nitrite to NO under hypoxia. Here, to test the hypothesis that a six- to five-coordinate heme iron transition might mediate the control of the nitrite reduction r...

The present study was designed to investigate the effect of captopril (20mg kg , i.p.) angiotensin 1 converting enzyme (ACE) inhibitor in learning and memory and on scopolamine (3mg kg , i.p), sodium nitrite 1 (75 mg kg , i.p.) and BN52021 (15mg kg , i.p.) a platelet activating factor (PAF) receptor antagonist, induced 1 1 amnesia in mice using water maze test. All the agents were administered ...

Elevated systolic pulmonary artery pressure (s-PAP, ≥35 mmHg) serves as an independent predictor of mortality in hemodialysis (HD) and diabetic (DM) patients. A polymorphism in the antioxidant Haptoglobin (Hp) gene has been shown to regulate the bioavailability of nitric oxide (NO), a major mediator of pulmonary vascular tone. We therefore set out to test the hypothesis that the Hp polymorphism...

Some eukaryotes, such as plant and fungi, are capable of utilizing nitrate as the sole nitrogen source. Once transported into the cell, nitrate is reduced to ammonium by the consecutive action of nitrate and nitrite reductase. How nitrate assimilation is balanced with nitrate and nitrite efflux is unknown, as are the proteins involved. The nitrate assimilatory yeast Hansenula polymorpha was use...

A new simple and highly sensitive spectrophotometric method for determining nitrogen dioxide in air was developed. The method is based on converting atmospheric nitrogen dioxide to nitrite ions within the IVL passive samplers used for samples collection. Acidifying nitrite ions with concentrated HCl produced the peroxynitrous acid oxidizing agent which was measured using 2, 2-azino-bis(3-ethyl ...

The study sought to test the hypothesis that co-administration of SNAP and cysteine or CapSNO and cysteine in normoglycaemic rats may further reduce glucose tolerance. An oral glucose tolerance was carried out and the plasma nitrite concentrations were measured and taken as the biochemical markers of in vivo NO formation. The results showed blood glucose levels were significantly elevated at fa...