The dimensions of local flaps are often limited by the vascular supply to the distal aspect of the flap. Distal flap necrosis occurs if the vascular supply is inadequate. The purpose of this study was to investigate the use of iontophoretic delivery of nitric oxide (NO) donors to a local skin flap model to improve the survival area of the flap. Thirty-two male Sprague-Dawley rats (300 g) were d...

The endogenous mediator of vasodilation, nitric oxide (NO), has been shown to be a potent radiosensitizer. However, the underlying mode of action for its role as a radiosensitizer - while not entirely understood - is believed to arise from increased tumor blood flow, effects on cellular respiration, on cell signaling, and on the production of reactive oxygen and nitrogen species (RONS), that ca...

nitric oxide is a short-lived mediator, which can be induced in a variety of cell types and produces many physiologic and metabolic changes in target cells. it is important in many biological functions and generated from l-arginine by the enzyme nitric oxide synthase. nitric oxide conveys a variety of messages between cells, including signals for vasorelaxation, neurotransmission and cytotoxici...

We have examined the responses of a population of 77 cells in the dorsal lateral geniculate nucleus (dLGN) of the anaesthetized, paralysed cat. Here the synthetic enzyme for the production of nitric oxide, nitric oxide synthase, is found only in the presynaptic terminals of the cholinergic input from the brainstem. In our hands, iontophoretic application of inhibitors of this enzyme resulted bo...

Oxidized low-density lipoprotein (ox-LDL) is assumed to be a major causal agent in hypercholesteraemia-induced atherosclerosis. Because the proliferation of lipid-loaden macrophages within atherosclerotic lesions has been described, we investigated the dependence of macrophage proliferation on the inhibition of inducible nitric oxide synthase (iNOS) by hypochlorite oxidized LDL. Ox-LDL induces ...

ischemia reperfusion injury (ir injury) is a common problem in clinical conditions. researches have frequently revealed that atp- sensitive potassium (katp) channels and nitric oxide plays a role in protection against ischemic injury in skeletal muscle. the present study aimed at evaluating the possible link between this two pathways. sixty-eight male wistar rats, were pretreated with saline, d...

Research during recent years has established nitric oxide as a unique signaling molecule that plays important roles in the regulation of the cardiovascular, nervous, immune, and other systems. Nitric oxide has also been implicated in the control of the secretion of hormones by the pancreas, hypothalamus, and anterior pituitary gland, and evidence is accumulating that it contributes to the regul...

In contrast to the apparent paucity of Mycobacterium tuberculosis response to reactive oxygen intermediates, this organism has evolved a specific response to nitric oxide challenge. Exposure of M. tuberculosis to NO donors induces the synthesis of a set of polypeptides that have been collectively termed Nox. In this work, the most prominent Nox polypeptide, Nox16, was identified by immunoblotti...

endothelial cell (HUVEC) and bovine aertic endothelial cell surface proteoglycans was significantly decreased by the incubation with SNAP andNOR4, poterrt nitric oxide (NO) donors. NO derived from lipopolysaccharide-stiinulated J774 A-1 cells also decreased the binding of EC-SOD to HUVEC, and this decrease was blocked by NG-nitro-L-arginine, a itric oxide synthase inhibiter. Furthermore. the de...