To engineer Nicotiana benthamiana to produce novel diterpenoids, we first aimed increase production of the diterpenoid precursor geranylgeranyl pyrophosphate (GGPP) by up-regulation key genes non-mevalonate (MEP) pathway sourced from Arabidopsis thaliana. We used transient expression evaluate combinations eight MEP plus GGPP synthase and a Jatropha curcas casbene (JcCAS) identify an optimal com...

Abstract Begomoviruses cause significant losses to a wide range of crops worldwide, and great progress has been made in characterizing some noncanonical proteins encoded by begomoviruses. In the present study, novel viral protein, C6, was detected Nicotiana benthamiana plants infected with tomato leaf curl China virus (ToLCCNV). Sequence analyses revealed that C6 ORF is on complementary strand ...

The model pathogen Pseudomonas syringae pv. tomato DC3000 causes bacterial speck in tomato and Arabidopsis, but Nicotiana benthamiana, an important model plant, is considered to be a non-host. Strain DC3000 injects approximately 28 effector proteins into plant cells via the type III secretion system (T3SS). These proteins were individually delivered into N. benthamiana leaf cells via T3SS-profi...

Nicotiana benthamiana is the most widely-used experimental host in plant virology. The recent release of the draft genome sequence for N. benthamiana consolidates its role as a model for plant-pathogen interactions. Quantitative real-time PCR (qPCR) is commonly employed for quantitative gene expression analysis. For valid qPCR analysis, accurate normalisation of gene expression against an appro...