We have isolated and sequenced a tRNAPhe gene from Neurospora crassa. Hybridization analyses suggest that trnaPhe is the only tRNA encoded on the cloned 5 kb DNA fragment. The tRNAPhe gene contains an intervening sequence 16 nucleotides in length located one nucleotide 3' to the anticodon position. The tRNAPhe coding region of Neurospora and yeast are 91% conserved, whereas their intervening se...

Cytosine methylation of DNA is an important epigenetic gene silencing mechanism in plants, fungi, and animals. In the filamentous fungus Neurospora crassa, nearly all known DNA methylations occur in transposon relics and repetitive sequences, and DNA methylation does not depend on the canonical RNAi pathway. disiRNAs are Dicer-independent small non-coding RNAs that arise from gene-rich part of ...

The genetics and biochemistry of tryptophan metabolism in the bread mold, Neurospora cras&a, have received considerable experimental attention during the past 20 years. By now, an impressive body of information has accumulated to the extent that the intimate details of the biosynthesise and degradation'-' of tryptophan in Neurospora are well known and also to the extent that the genetics of thi...

It is now believed that the amino groups of isoleucine and valine are accepted by their respective carbon chains during biosynthesis via a transamination. Most of the evidence for this hypothesis has been derived from experiments on isoleucineless and valineless mutants of Escherichia coli and Neurospora crassu (l-4). It is not positively established whether this transamination step can be medi...

Blue light-induced absorbance changes were measured from differentially centrifuged membrane fractions from dark-grown coleoptiles of Zea mays L., and mycelia from an albino mutant of Neurospora crassa. Actinic irradiation caused changes in absorbance consistent with a flavinmediated reduction of a b-type cytochrome. Both corn and Neurospora showed similar light-minus-dark difference spectra, d...

Neurospora tetrasperma is a pseudohomothallic filamentous ascomycete that has evolved from heterothallic ancestors. Throughout its life cycle, it is predominantly heterokaryotic for mating type, and thereby self-fertile. However, studies of N. tetrasperma have revealed the occasional production of self-sterile asexual and sexual spores of a single-mating type, indicating that it can be function...

In Neurospora crassa, the interactions between products of the frequency (frq), frequency-interacting RNA helicase (frh), white collar-1 (wc-1), and white collar-2 (wc-2) genes establish a molecular circadian clockwork, called the FRQ-WC-Oscillator (FWO), which is required for the generation of molecular and overt circadian rhythmicity. In strains carrying nonfunctional frq alleles, circadian r...

The poky (mi-1) strain of Neurospora crassa is a and the mass ratio of large to small subunit RNA useful test organism in which to evaluate methods is -10 :1 (1) . Nonetheless, mitochondrial protein for isolating mitochondrial ribosomes actively synthesis can be observed in poky (2) and cytoengaged in protein synthesis . In poky mitochondria chrome oxidase which is in part synthesized by there ...

In this paper we report on the reactions and enzyme components of transsulfuration in Neurospora crassa, Saccharomyces cerevisiae, Escherichia co& and Salmonella typhimurium, and show that the process is reversible in the higher fungi, and irreversible, in the direction opposite to that of mammalian tissues, in the bacteria. Recently, we have reported the isolation of two cystathionine cleavage...