objective: schizophrenia is a complex widespread neuropsychiatric disorder. this illness encompasses a complex debilitating mental disorder causing illusion, delusion, disturbed relationship, low motivation and decline of emotion. viral infection of the brain including borna disease virus (bdv) may play a role in transient or permanent neurological and behavioral abnormalities. this role of bor...

Detection of Coxiella burnetii, the etiologic agent of Q fever, is important for diagnosis of Q fever. PCR-based methods have been widely used for the detection mostly because isolation of C. bumetii is time-consuming. Recent reports showed that PCR-positive rates of Q fever infection widely differed. We have evaluated the PCR and nested PCR assays currently used in Japan. The nested PCR assay ...

PURPOSE Reverse-transcriptase PCR (RT-PCR) assays for carcinoembryonic antigen (CEA) have been described to identify lymph node micrometastases. These assays are not quantitative and can be confounded by false-positive results. The purpose of this study was to determine whether quantification of CEA in lymph nodes could more readily identify clinically relevant groups. EXPERIMENTAL DESIGN Spe...

conclusions in this study, snpcr was specific and more sensitive than the chromogenic medium chromagar™ candida for the detection of candida spp. insofar as it failed to identify only a few isolates. materials and methods we used snpcr with universal and species-specific primers to detect candida species in the culture of clinical isolates. by using universal primers, we carried out the amplifi...

pneumocystis jirovecii causes pneumocystis pneumonia (pcp) in immunocompromised patients with a high rate of morbidity and mortality. colonization with this fungus may stimulate pulmonary inflammation or lead to pcp in susceptible patients. the epidemiology of this infection and routs of its transmission has poorly studied in iran. we examined pneumosystis colonization in patients with various ...

leptospirosis is a zoonosis of worldwide distribution, caused by leptospira interrogans and is considered as an emerging global public health problem. transmission usually results from direct or indirect exposure to the urine or other body fluids of leptospiruric animals which may become a source of infection for human or other animals. having a humid climate with plenty of annual rainfall, gui...

a rapid and sensitive reverse transcription polymerase chain reaction (rt-pcr) and nested-pcr were used to detect bovine viral diarrhea virus 1 (bvdv-1) in bull semen. selected primers could amplify a part of the 5´utr of the bvdv genome. a 294 bp dna fragment was amplified and specificity of the results was confirmed by direct sequencing of the pcr product. prior to rna extraction, the seminal...