The genetic stability of normal and neoplastic lymphocytes was compared by using base-line mutation frequency and mutation rate/cell generation. Mutations at the hypoxanthine-guanine phosphoribosyltransferase locus were studied by enumerating thioguanine-resistant cells in a clonogenic assay. The base-line ("spontaneous") mutation frequency was 1.52 X 10(-6), 6.38 X 10(-6), and 1.06 X 10(-6) fo...

was induced by carcinogen treatment. Many cell types in culture undergo a process of neoplastic transformation spon taneously, i.e., in the absence of a known carcinogenic insult (31). During this transformation process, aneuploid cell lines arise that are often nontumorigenic in animals when injected as a cell suspension. These lines are generally termed “nor mal' â€ãnd are frequently used a...

background: essential oils are found to have multiple active components which can show in vitro cytotoxic action against various cancerous cell lines. this study reports the in vitro cytotoxic effects of the essential oil from artemisia absinthium l. (asteraceae) growing wild in iran. methods: water-distilled essential oil of a. absinthium collected from ardabil, north-western iran, was examine...

The non-Hodgkin's lymphomas (NHL) are a diverse group of human lymphoid neoplasms that have long presented pathologists with formidable diagnostic challenges. These tumors of the immune system are thought to represent neoplastic transformations of most of the recognized stages in T and B lymphocyte ontogeny. Lymphoma cells, however, often simulate their normal lymphocytic counterparts both morp...

Studies using transgenic and knockout mice have demonstrated that particular cytokines influence lung tumor growth and identified prostaglandin E2 (PGE2), prostacyclin (PGI2) and nitric oxide (NO) as critical mediators of this process. PGE2 and NO were pro-tumorigenic while PGI2 was antitumorigenic. We describe herein an in vitro experimental approach to examine interactions among cytokines, pr...

Aims-To establish two cell lines from a primary duodenal adenocarcinoma; to describe the morphological, growth, ploidy, and immunophenotypic characteristics of these cell lines.Methods-The cell lines, designated DAC/S and DAC/E, were characterised using both in vitro and in vivo cell culture techniques, light and electron microscopy, immunocytochemistry, and FACS analyses.Results-Both cell line...

The membrane glycopeptides of normal and chemically transformed malignant cell lines derived from rat kidney were investigated by gel permeation chromatography. The cell lines, which were metabolically labeled with i_-{5,6-3H]fucose or o-[63H]glucosamine, were derived from rats treated in vivo with a carcinogenic dose of dimethylnitrosamine to induce both mesenchymal and cortical epithelial tum...