Transport between the nucleus and cytoplasm involves both stationary components and mobile factors acting in concert to move macromolecules through the nuclear pore complex. Multiple transport pathways requiring both unique and shared components have been identified. In the past 18 months, new findings have shed light on the nature of some of the mobile components of these pathways. New recepto...

nipple discharge (nd) can be the earliest presenting symptom of breast cancer. we hereby present two cases of breast cancer with no palpable mass manifesting as isolated nd, which was whitish in color. in both cases, cytology of the discharge revealed highly pleomorphic cells indicating a high grade malignancy. mammography showed diffuse, extensive microcalcifications. simple mastectomy with ax...

Currents flowing through single dihydropyridine-sensitive Ca2+ channels were recorded from cell-attached patches on C2 myotubes. In the presence of dihydropyridine agonist to prolong the duration of single-channel openings, adding micromolar concentrations of lanthanum (La), cerium (Ce), neodymium (Nd), gadolinium (Gd), dysprosium (Dy), or ytterbium (Yb) to patch electrodes containing 110 mM Ba...

INVESTIGATION OF PHYSIOCHEMICAL PROPERTIES OF A NOVEL GRADIENTCALCIUM POLYPHOSPHATE BONE SCAFFOLD AND ITS INFLUENCE ONCELLULAR BEHAVIORByLIANG CHENDecember 2013Advisor: Dr. Weiping RenMajor: Biomedical EngineeringDegree: Master of ScienceA good designed bone scaffold is crucial to bone tissue engineering. We have developedand characterized a novel gradien...

Nuclear pore complexes (NPCs) are enormous macromolecular structures that mediate the active exchange of proteins and RNPs between the nucleus and cytoplasm. Recent work has resulted in a windfall of identified NPC polypeptides, many with unique sequences. Several of the proteins have been shown to be part of extended cytoplasmic and nucleoplasmic NPC filaments. Biochemical, structural and gene...

FG nucleoporins (Nups) are the class of proteins that both generate the permeability barrier and mediate selective transport through the nuclear pore complex (NPC). The FG Nup family has 11 members in Saccharomyces cerevisiae, and the study of mutants lacking different FG domains has been instrumental in testing transport models. To continue analyzing the distinct functional roles of FG Nups in...

We describe a method to genetically manipulate Chaetomium thermophilum, a eukaryotic thermophile, along with various biochemical applications. The transformation method depends on a thermostable endogenous selection marker operating at high temperatures combined with chromosomal integration of target genes. Our technique allows exploiting eukaryotic thermophiles as source for purifying thermost...

Eukaryotic cells have developed a series of highly controlled processes of transport between the nucleus and cytoplasm. The present review focuses on the latest advances in our understanding of nucleocytoplasmic exchange of molecules in yeast, a widely studied model organism in the field. It concentrates on the role of individual proteins such as nucleoporins and karyopherins in the translocati...

conclusions our study showed that the triple therapy of nd: yag and nd: yag + co2 fractional and hydroquinone cream had high efficiency and low morbidity compared to twofold treatment with nd: yag and nd: yag + co2 fractional. results seventeen patients with melasma (14 females and three males) with mean (± sd) age of 38.47 (± 6.29) years (range 29 - 53) were included in this study. the only si...