A modified ELISA procedure is described which permits selective detection of monoclonal antibodies reactive with the native antigen. The essence of the method is the use of immobilization methods that do not result in denaturation of the antigen during adsorption to the microtiter plates. This was accomplished by using polyclonal antibodies, adsorbed to the wells of the microtiter plate, as the...

vp2 gene coding region of a vaccinal strain (d78) of infectious bursal disease virus (ibdv) was clonedin a eukaryotic expression vector, psec tag2a. the gene was placed downstream of ig κ chain leadersequence, under the control of human cytomegalovirus (hcmv) immediate early enhancer and promoter. theconstruct psec tag2a-vp2 was transfected in cos-7 cell line and the expression and secretion of...

Structure determination of adjuvant-coupled antigens is essential for rational vaccine development but has so far been hampered by the relatively low antigen content in formulations and their heterogeneous composition. Here we show that magic-angle spinning (MAS) solid-state NMR can be used to assess structure influenza virus hemagglutinin stalk long alpha helix antigen, both its free, unformul...

Please cite this article in press as: Atif, Y. Conve http://dx.doi.org/10.1016/j.chb.2012.07.026 Today’s college students have grown up with technology. These digital natives typically gravitate toward group activities in technology embedded social contexts. However, despite this multidimensional evolution, little has changed in conventional classrooms where they build their education experienc...

This chapter analyzes the adequacy of two university education models according to their Information Communications Technology (ICT) usage: One model is based on the use of ICT as a cornerstone and a cohesive element of the teaching-learning process, whereas the other considers ICT a mere teaching support tool. The preliminary results obtained from a review of case studies reveal that ICT use i...