We automated two procedures for determination of urinary N-acetyl-beta-D-glucosaminidase (NAG; EC 3.2.1.30) concentrations and evaluated their reliability for detecting drug-induced tubular damage in children receiving cisplatin, methotrexate, or ifosfamide. Results for 174 patient specimens correlated well (r = 0.98), but NAG concentrations determined by the m-cresolsulfonphthaleinyl (MCP) pro...

NAG (N-acetyl-L-glutamate), the essential allosteric activator of the first urea cycle enzyme, CPSI (carbamoyl phosphate synthetase I), is a key regulator of this crucial cycle for ammonia detoxification in animals (including humans). Automated cavity searching and flexible docking have allowed identification of the NAG site in the crystal structure of human CPSI C-terminal domain. The site, a ...

The aim of the present study was to evaluate the anticancer effects of chitooligosaccharides (COS) and N-acetyl-D-glucosamine (NAG), as well as to investigate the possible mechanisms involved. MTT assay and flow cytometry were used to evaluate the effect of various concentrations of COS and NAG on the proliferation and differentiation of peripheral blood mononuclear cells (PBMCs). In addition, ...

N-acetyl glucosamine, the monomer of chitin, is an abundant source of carbon and nitrogen in nature as it is the main component and breakdown product of many structural polymers. Some bacteria use N-acyl-L-homoserine lactone (AHL) mediated quorum sensing (QS) to regulate chitinase production in order to catalyze the cleavage of chitin polymers into water soluble N-acetyl-D-glucosamine (NAG) mon...

To study the process of chronic peritoneal inflammation from sterile solutions, we established an animal model to link structural changes with solute and water transport. Filtered solutions containing 4% N-acetylglucosamine (NAG) or 4% glucose (G) were injected intraperitoneally daily in 200- to 300-g rats and compared with controls (C). After 2 mo, each animal underwent transport studies using...

Nanocrystalline silver (nAg) and Manuka honey (MH) dressing have increasing popularity for treating diabetic foot ulcer (DFU). This study was an open-label randomized controlled trial with three parallel groups' design in examining the preliminary effectiveness of nAg against MH and conventional dressing in healing DFU in terms of ulcer healing, ulcer infection, and inflammation. 31 participant...

The glycosidase-recognizing N-acetylglucosamine terminal residue, N-acetylglucosaminidase (NAG), has been repetitively implicated in fertilization. Nevertheless, its role in the multiple steps comprising this process is a matter of debate because it has been involved in zona pellucida (ZP) binding and penetration and polyspermy block. In this study, the involvement of NAG during sperm interacti...

The contribution of the form silver nanomaterials (nAg) towards toxicity in aquatic organisms is not well understood. purpose this study was to examine various structures (sphere, cube and prism) nAg Dreissena bugensis mussels. Mussels were exposed increasing concentrations polyvinyl-coated same size for 96 h at 15 °C. They then analyzed biophysical changes cytoplasm (viscosity, protein aggrega...

The analytical, within-subject, and between-subject components of variation of N-acetyl-beta-D-glucosaminidase (NAG) were estimated from duplicate assays of 10 timed first morning and 10 untimed urine specimens collected from each of 15 ostensibly healthy individuals. Results were expressed in terms of activity, NAG/creatinine ratio, and excretion rate. Current analytical methods can achieve de...