نتایج جستجو برای: nadph

تعداد نتایج: 21368  

Journal: :Journal of molecular and cellular cardiology 2005
Gina Sánchez Zully Pedrozo Raúl J Domenech Cecilia Hidalgo Paulina Donoso

We have shown previously that electrically induced tachycardia effectively produces myocardial preconditioning. Among other effects, tachycardia increases calcium release rates in microsomal fractions enriched in sarcoplasmic reticulum (SR) isolated from dog cardiac ventricular muscle. Here, we report that preconditioning tachycardia increased twofold the NADPH oxidase activity of isolated SR-e...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1991
B T Hope G J Michael K M Knigge S R Vincent

NADPH diaphorase histochemistry selectively labels a number of discrete populations of neurons throughout the nervous system. This simple and robust technique has been used in a great many experimental and neuropathological studies; however, the function of this enzyme has remained a matter of speculation. We, therefore, undertook to characterize this enzyme biochemically. With biochemical and ...

Journal: :The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1999
R G Wehby M E Frank

We had previously shown NADPH diaphorase activity in fixed tissue slices of the insular cortex of the Syrian golden hamster (Mesocricetus auratus). The objective of this work was to determine the chemical identity of agents responsible for the observed NADPH diaphorase activities. Three different enzymatic NADPH diaphorase activities were distinguished in the insular cortex. (a) The activity se...

Journal: :Molecular human reproduction 2001
S C Richer W C Ford

It has been suggested that human spermatozoa contain an NADPH oxidase that could generate reactive oxygen species involved in signalling pathways to promote fertility. The proposal depends on observations that the addition of NADPH to purified human spermatozoa stimulates chemiluminescence by the superoxide (O2-) probe, lucigenin. We confirmed these observations, but demonstrated that lucigenin...

Journal: :General physiology and biophysics 2002
D Kluchová R Klimcík P Kloc

The NADPH-diaphorase (NADPH-d) staining method is widely used in the investigation of both the central and peripheral nervous systems. Neuronal nitric oxide synthase (nNOS) has previously been shown to be responsible for the NADPH-d activity in neurons. However, NADPH-d activity does not always fully represent the enzyme nNOS. We investigated the distribution of NADPH-d activity and nNOS protei...

Journal: :American journal of physiology. Cell physiology 2009
Caroline N White Gemma A Figtree Chia-Chi Liu Alvaro Garcia Elisha J Hamilton Karin K M Chia Helge H Rasmussen

The sarcolemmal Na(+)-K(+) pump, pivotal in cardiac myocyte function, is inhibited by angiotensin II (ANG II). Since ANG II activates NADPH oxidase, we tested the hypothesis that NADPH oxidase mediates the pump inhibition. Exposure to 100 nmol/l ANG II increased superoxide-sensitive fluorescence of isolated rabbit ventricular myocytes. The increase was abolished by pegylated superoxide dismutas...

Journal: :Biochemical Society transactions 2010
Allan G Rasmusson Sabá V Wallström

NADPH and NADH mediate reductant flow between cellular processes, linking central carbon and energy metabolism with intermediary metabolism, stress defence and development. Recent investigations have revealed paths of functional interactions, and have suggested that mitochondrial NADPH oxidation, especially together with the oxidative pentose phosphate pathway, is an important regulator of the ...

Journal: :Drug metabolism and disposition: the biological fate of chemicals 2009
Christopher S Mazur John F Kenneke Michael-Rock Goldsmith Cather Brown

Carbonyl containing xenobiotics may be susceptible to NADPH-dependent cytochrome P450 (P450) and carbonyl-reduction reactions. In vitro hepatic microsome assays are routinely supplied NADPH either by direct addition of NADPH or via an NADPH-regenerating system (NRS). In contrast to oxidative P450 transformations, which occur on the periphery of a microsome vesicle, intraluminal carbonyl reducti...

Journal: :The Journal of neuroscience : the official journal of the Society for Neuroscience 2000
K M Noh J Y Koh

Zinc overload may be a key mechanism of neuronal death in acute brain injury. We have demonstrated previously that zinc overload neurotoxicity involves protein kinase C (PKC)-dependent rises in intracellular levels of reactive oxygen species (ROS). However, the cascade linking PKC activation to ROS generation in cultured cortical neurons has been unknown. A recent study has demonstrated that RO...

2015
Yushi Ishibashi Shinsuke Kasa Masatsugu Sakamoto Nozomi Aoki Kyohei Kai Takashi Yuasa Atsushi Hanada Shinjiro Yamaguchi Mari Iwaya-Inoue Chengdao Li

Reactive oxygen species (ROS) promote the germination of several seeds, and antioxidants suppress it. However, questions remain regarding the role and production mechanism of ROS in seed germination. Here, we focused on NADPH oxidases, which produce ROS. After imbibition, NADPH oxidase mRNAs were expressed in the embryo and in aleurone cells of barley seed; these expression sites were consisten...

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