The purified 15-lipoxygenase from rabbit reticulocytes is capable of oxidizing NADH in the presence of linoleic acid and oxygen. This co-oxidation proceeds at a rate that amounts to approx. 7% of linoleic acid oxygenation rates. Although NADH inhibits the lipoxygenase reaction with linoleic acid as substrate (46% inhibition at 0.2 mM NADH), the reaction specificity of the enzyme was not altered...

Oxidative demethylation of 4-methyl sterols by rat liver microsomes has been shown to be inhibited by low levels of NADH. Using snake venom-treated microsomes to remove endogenous NADH-utilizing activities, we observed inhibition of overall demethylation at concentrations of NADH as low as 2.5 p ~ . At 20 p~ NADH, overall demethylation had been reduced to 60% of optimal activity. In the presenc...

background: fasciola hepatica and f. gigantica are the causative agents of fasciolosis in domestic animals and humans. based on the morphometric criteria, differential diagnosis between them is problematic. in addition, intermediate forms of fasciola have been found in iran, which makes the differentiation more difficult. the aim of the present study was to provide molecular evidence for the ex...

We have developed genetically encoded fluorescent sensors for reduced nicotinamide adenine dinucleotide (NADH), which manifest a large change in fluorescence upon NADH binding. We demonstrate the utility of these sensors in mammalian cells by monitoring the dynamic changes in NADH levels in subcellular organelles as affected by NADH transport, glucose metabolism, electron transport chain functi...

We studied the effects of mexidol (3-oxy-6-methyl-2-ethylpiridine succinate) on antioxidant glutathione system in rat brain mitochondria experimental Parkinson’s disease induced by rotenone administration. Wistar rats were divided into following groups 6 each: I - intact (control); II (3 mg/kg per day) was injected subcutaneously for 2 weeks; III after intoxication, (50 intraperitoneally weeks....

The reduced coenzyme NADH plays a central role in mitochondrial respiratory metabolism. However, reports on the amount of free NADH in mitochondria are sparse and contradictory. We first determined the emission spectrum of NADH bound to proteins using isothermal titration calorimetry combined with fluorescence spectroscopy. The NADH content of actively respiring mitochondria (from potato tubers...

The NADH oxidase-peroxiredoxin (Prx) system of Amphibacillus xylanus reduces hydroperoxides with the highest turnover rate among the known hydroperoxide-scavenging enzymes. The high electron transfer rate suggests that there exists close interaction between NADH oxidase and Prx. Variant enzyme experiments indicated that the electrons from β-NADH passed through the secondary disulfide, Cys128-Cy...

We developed an analytical reverse-phase high-performance liquid chromatographic procedure for rapid assessment of the purity of NADH. The method completely separates adenosine monophosphate and adenosine diphosphoribose from NADH. By use of this analytical technique we found that preparative chromatography on DEAE-cellulose gives NADH that is free from adenine nucleotides as well as other impu...

Background: Aldo-keto oxidoreductase (AKR) inhibitors could reverse the resistance of several cancer cells to cis-platin, but their role in remains unclear.Methods: We verified difference AKR1Cs expression by Western blot, RNA sequencing and qRT-PCR. The differences were analyzed inferred. Use Assay NADH NAD+ content verify inference. Docking experience was used affinity between MPA, MCFLA, MLS...