Normal erythrocytes were subject to swelling and some haemolysis when suspended in a NaCl-formaldehyde-Na(2)EDTA diluent of pH 4.9 for routine blood-cell counting in the Coulter counter. A phosphate-buffered saline of pH 7.4 was substituted and has proven satisfactory for total erythrocyte counts. Polyvinyltoluene latex was unsuitable for volume calibration of the counter, because of aggregatio...

The mechanisms of ion movement across the apical membrane of the colon have previously been investigated only in intact tissue. To investigate these mechanisms directly, we have undertaken the isolation and characterization of the apical brush-border membrane of the rabbit descending colon. The purification protocol consists of an initial isolation of single epithelial cells after dissociation ...

In circulation, platelets may come into contact with both exogenous (cardiac glycoside treatment) and endogenously produced inhibitors of Na+/K(+)-ATPase. We examined whether blocking of platelet Na+/K(+)-ATPase by ouabain results in generation of procoagulant activity. It was shown that an in vitro treatment of platelets with ouabain (20-200 microM for 20 to 60 min) is associated with an intra...

introduction : the aim of this in vitro study was to evaluate the effect of smear layer on the push-out bond strength of white mineral trioxide aggregate (wmta) with and without disodium hydrogen phosphate (na 2 hpo 4 ). materials and methods: dentin discs with standard cavities were obtained from extracted human single-rooted teeth and divided to 4 groups ( n =15) according to the irrigation r...

The aim of this study was to compare the influence a final rinse with and without passive ultrasonic irrigation (PUI), using 17% ethylenediaminetetraacetic acid (EDTA) or 10% citric (CA), 2.5% sodium hypochlorite (NaOCl) PUI, on sealing ability root canal fillings. Root instrumentation performed in single-rooted premolars NaOCl as irrigant. Subsequently, roots were randomly allocated into four ...

Through the esterification of cellulose (Cell) with ethylenediaminetetraacetic dianhydride, acid-functionalized (Cell-EDTA) was conveniently obtained. Furtherly a novel and efficient Cell-EDTA-supported palladium catalyst (Pd@Cell-EDTA) easily prepared via coordination Cell-EDTA PdCl2. The as-prepared Pd@Cell-EDTA fully characterized by inductively coupled plasma atomic emission spectrometry, X...

Experiments with plasma membrane from rat liver have been interpreted as showing that the activity of the (Na++K+)-dependent ATPase* is subject to modulation by the action of hormones, such as L-adrenaline (epinephrine) and insulin, through the latter's effects on adenylate cyclase activity and consequent changes in cyclic AMP concentration (Luly et af. , 1972; Tria et af . , 1974; Luly & Emmel...