Abstract: The non-tuberculous mycobacteria (NTM) are ‘atypical mycobacteria’ belonging to species other than those classified in the Mycobacterium tuberculosis complex. NTM have been reported cause cross-reactive immune responses that interfere with diagnosis of both livestock and wildlife considered as potential pathogens for animals humans. present study was conducted detection non- tuberculo...

background: genital tuberculosis (gtb) is an important cause of female infertility, especially in developing countries. the positive results of polymerase chain reaction (pcr) in endometrial gtb in the absence of tubal damage raise the possibility of the detection of sub-clinical or latent disease, with doubtful benefits of treatment. objective: to evaluate the mycobacterium tuberculosis infect...

Differentiation of M. tuberculosis and M leprae by polymerase chain reaction (PCR), when acid-fast bacilli (AFB) were present in sputum from patients at Anandaban hospital, was carried out. Thirty sputum samples microscopy positive for AFB were collected and were subjected to culture. Bacterial DNA was extracted and PCR was performed using primers specific for Mycobacterium tuberculosis and Myc...

The polymerase chain reaction (PCR) was used to identify mycobacterial DNA sequences in uncultured clinical specimens. Two oligonucleotide primers derived from the sequence of a gene that codes for the 65-kilodalton antigen of Mycobacterium tuberculosis amplified DNA from all 11 species of mycobacteria tested. Amplified DNAs of nontuberculosis mycobacteria were found to be approximately 20 to 4...

The earliest known case of human tuberculosis in Britain dates to the middle period of the Iron Age, approximately 2,200 years before present. Bone lesions on the spine of a male skeleton excavated at Tarrant Hinton in Dorset, United Kingdom, show evidence of Pott's disease and are supported by molecular evidence of Mycobacterium tuberculosis complex DNA amplified by IS6110 PCR (19). In the pre...

Tuberculosis control is a priority for the Ministry of Health policies in Brazil. In the present work, the detection of Mycobacterium tuberculosis by the Polymerase Chain Reaction (PCR) was standardized, and the laboratory diagnosis of pulmonary tuberculosis was evaluated comparing baciloscopy, culture and PCR tests. The study was carried out with 117 sputum samples from different patients susp...

tuberculosis is one of the most important infectious diseases and a major cause of morbidity and mortality in developing countries. multi drug resistant tuberculosis is a new threat, which can result in a high case fatality rate. this study was conducted to define the incidence of multidrug resistant tuberculosis in kermanshah. ­antibiogram was performed on­­ pcr-confirmed isolates of mycobacte...

Identification and characterization of the Mycobacterium tuberculosis strains are important for clinical and therapeutic management of tuberculosis. Real-time PCR with a high-resolution melt assay was found to improve the diagnostic process. The assay includes differentiation between M. tuberculosis and Mycobacterium bovis based on one single-nucleotide polymorphism (SNP) in the narGHJI and oxy...