In the presence of iron (Fe), dissolved organic matter (DOM) may bind considerable amounts of arsenic (As), through formation of Fe-bridged As-Fe-DOM complexes and surface complexation of As on DOM-stabilized Fe-colloids (collectively referred to as As-Fe-DOM complexation). However, direct (e.g., chromatographic and spectroscopic) evidence and fundamental kinetic and stability constants have be...

A pumped bed-membrane bioreactor (PB-MBR) combining powdered activated carbon (PAC) adsorption with bacterial degradation was studied for the purification of tap water, spiked with 2 mg/l of phenanthrene, to sub-ppm levels. The feedwater was supplemented with nitrogen and phosphorous to reach a COD:N:P ratio of 100:10:1. PAC concentration was maintained at 5 g/l. Polyacrylonitrile (PAN) flat-sh...

The aim of this work was the purification and identification of the major angiotensin converting enzyme (ACE) inhibitory peptides produced by enzymatic hydrolysis of a protein concentrate recovered from a cuttlefish industrial manufacturing effluent. This process consisted on the ultrafiltration of cuttlefish softening wastewater, with a 10 kDa cut-off membrane, followed by the hydrolysis with ...

Prediction of the dynamic crossflow ultrafiltration rate of a protein solution such as milk poses a complex non-linear problem a...

The performance of thin-film composite (TFC) membranes was related to the properties of support layers. In this study, polysulfone (PSf) membranes formed by the phase inversion process under various concentrations of PSf and polyvinylpyrrolidone (PVP) additive were used as support layers to prepare polyamide (PA) TFC membranes by the interfacial polymerization (IP). The effects of PSf and PVP c...

Iodine preparations for external use are recommended for treating pressure ulcers with manifestations of infection and necrosis. These ulcers abundantly produce wound exudates, which could be absorbed by water-soluble base. In this study we aimed to improve the previously reported methodologies for water absorption and new methodologies were developed in Franz diffusion cell with 100kDa molecul...

Scientists recently reported the unexpected detection of unknown or poorly studied bacterial diversity in groundwater. The ability to uncover this neglected biodiversity mainly derives from technical improvements, and the term "microbial dark matter" was used to group taxa poorly investigated and not necessarily monophyletic. We focused on such under-investigated microbial dark matter of drinki...

Silk fibroin can be made into various forms of biocompatible medical materials, including hydrogel due to its excellent properties. Here, we report a novel method for the preparation of electropolymerized silk fibroin hydrogel membrane (ESFHM), which is formed on a nanoporous film as a barrier using a homemade device at a higher DC voltage. Regenerated silk fibroin solution in Tris buffer (pH 6...

Cloning, expression and purification The gene coding for the SP lyase from Geobacillus thermodenitrificans (GTNG_2348) and the C140A mutant were cloned in our previous study. [1] The C140A/S76C mutant for this study was generated by site-directed mutagenesis (by QuickChange PCR mutagenesis) using the pET-M11-C140A mutant vector as template and the following primers with the modified bases indic...

A major gap in understanding nanomaterials behaviour the environment is a lack of reliable tools to measure their available concentrations. In this research we use diffusive gradients thin films (DGT) for measuring concentrations zinc oxide nanoparticles (ZNO NPs) soils. Available nanoparticle were assessed by difference, using paired DGT devices with and without 1000 MWCO dialysis membranes ex...