mtt assay is designed for spectrophotometric quantification of cell growth and viability without using the radiactive isotopes. the aim of this study is com¬parison of ltt and mtt as sensitive poliferation assays in immunodeficient pa¬tients. 20 immunodeficient and 20 healthy subjects were selected in this study. all of them had normal lymphocytes count, normal cd3 and negative dth reac¬tion to...

<p style="text-align: justify;">This study aimed to find the Classical Test Theory (CTT) and Modern (MTT) item parameters of Differential Aptitude (DAT) examined their comparability them. The being studied are difficulty level discrimination index. 5.024 data result sub-test DAT were documented by Department Psychology Guidance Counselling bureau. parameter classical modern test items was...

MTT assay is commonly used to assess the cellular cytotoxicity caused by anticancer drugs in glioblastomas. However, there have been some reports insisting that MTT assay exhibited non-specific intracellular reduction of tetrazolium which led to underestimated results of cytotoxicity. Here, we examine whether or not MTT assay can lead to incorrect information regarding alcohol-induced cytotoxic...

Humans have the abilities to perceive, produce, and synchronize with a musical beat, yet there are widespread individual differences. To investigate these abilities and to determine if a dissociation between beat perception and production exists, we developed the Harvard Beat Assessment Test (H-BAT), a new battery that assesses beat perception and production abilities. H-BAT consists of four su...

Background: Evaluation of cell viability is momentous in pharmacologic and oncological research. Cell viability evaluation determines cell sensitivity and consequently treatment outcome. Various methods are available to determine cell survival. Each of these methods evaluates different endpoints. Accordingly, determining the correlation between these methods is important. In this study, in orde...

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The lymphocyte stimulation test (LST) is one of the most useful laboratory tests for the identification of allergy to a specific drug. The present study was conducted to examine utility of the LST using the [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) assay as a method for diagnosing drug allergy. The basic experimental conditions for the MTT assay were determined by u...