نتایج جستجو برای: mtt formazan

تعداد نتایج: 15467  

Journal: :American journal of physiology. Heart and circulatory physiology 2005
F A DeLano R Balete G W Schmid-Schönbein

One mechanism for organ damage in individuals with arterial hypertension may be due to oxygen free radical production. This study was designed to localize free radicals in a microvascular network of mature spontaneously hypertensive rats (SHRs) and normotensive Wistar-Kyoto (WKY) rats. Because glucocorticoids play a role in pressure elevation of SHRs, we investigated their role in microvascular...

Journal: :Neuro endocrinology letters 2014
Zdeněk Zídek Petr Jansa Eva Kmoníčková

OBJECTIVES Formation of formazan is a commonly used measure of cytotoxicity of compounds. It is a product of reduction of tetrazolium salts such as 4-[3- (4-iodophenyl)-2- (4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate (WST-1) and 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride. The extent of substrates reduction reflects the activity of enzymes succinate dehydrogenase...

Journal: :Indian journal of medical microbiology 2006
S N Arseculeratne D N Atapattu P Balasooriya R Fernando

No data exists on the activity of biocides (antiseptics and disinfectants) on Rhinosporidium seeberi that causes rhinosporidiosis in humans and animals. On account of the inability to culture R. seeberi, in vitro, dyes were used to assess the morphological integrity and viability of biocide-treated endospores that are considered to be the infective stage of this pathogen. Evan's Blue (EvB) iden...

Journal: :Kumamoto Igakkai zasshi. The Journal of the Kumamoto Medical Society 1967
A Mizutani

For the application of Glenner's tetrazolium method to the electron histochemical demonstration of monoamine oxidase some in vitro examination were carried out with special referance to indentification and efflux of formazan and intracellular localization of the enzyme in the locus coeruleus of the rat brain was studied. The results of examinations in vitro are summerized as follows: 1. Formaza...

A. Mirmoini F. Bagheri F. Mojab N. Rahimifard SR. Pakzad*

Background and objectives: Evaluating the safety of pharmaceutical products by in vitro methods like cell culture, and in vivo tests on animals, is one of the regulatory steps in development of drugs which is called the preclinical stage. If acceptable results are obtained, the drug can be used in clinical studies. In this study, we have investigated the cytot...

Journal: :Dental materials : official publication of the Academy of Dental Materials 1992
J C Wataha R G Craig C T Hanks

Previous studies have utilized in vitro alloy cytotoxicity tests to evaluate dental casting alloys. The purposes of this study were to: (1) evaluate the precision of the optical density and visual tests previously used, (2) evaluate a new test measuring absorbance of solubilized formazan dyes, and (3) test the correlation between these tests for cytotoxicity. Balb/c 3T3 cells were plated in 24-...

2007
Asti Meizarini

The requirements for dental material include not toxic, not irritant, no carcinogenic potential, nor cause an allergic response with the use in oral cavity. The cyanoacrylate restoration material has certain substance that can be toxic. Because of the ratio amount of powder and liquid is not known, it can lead the restoration more toxic. The purpose of this study was to know the cytotoxicity of...

2014
Hermann Agis Amy Collins Andrei D. Taut Qiming Jin Laura Kruger Christoph Görlach William V. Giannobile

Biodegradable collagen scaffolds are used clinically for oral soft tissue augmentation to support wound healing. This study sought to provide a novel ex vivo model for analyzing healing kinetics and gene expression of primary human gingival fibroblasts (hGF) within collagen scaffolds. Sponge type and gel type scaffolds with and without platelet-derived growth factor-BB (PDGF) were assessed in a...

2015
Acharya Balakrishna M. Hemanth Kumar

Ayurvedic system of medicine is using Berberis aristata and Curcuma longa herbs to treat different diseases including cancer. The study was performed to evaluate the synergetic anticancer activity of Berberine and Curcumin by estimating the inhibition of the cell proliferation by cytotoxicity assay using MTT method on specified human cell lines (A549, Hep-G2, MCF-7, Jurkat, and K562). All the c...

2014
Krishna Kolan

The pore geometry of bone scaffolds, intended for use in bone repair or replacement, is one of the most important parameters in bone tissue engineering. It affects not only the mechanical properties of the scaffolds but also the amount of bone regeneration after implantation. Scaffolds with five different architectures and four porosity levels were fabricated using borate bioactive glass (13–93...

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