Quantitative real-time or kinetic RT-PCR is increasingly used for the quantification of specific mRNA targets, especially in clinical applications. To quantify the mRNA of cytokines and their receptors, which play important roles in the pathogenesis of autoimmune diseases such as multiple sclerosis, we have developed quantitative two-step RT-PCR assays for IL-4, IL-4R, IFN-gamma, IFN-beta, and ...

the construction industry is a major consumer of new materials. given that material production for construction work accounts for a significant percentage of all energy consumed nationally in newly developing countries, it becomes vital that the construction industry strives to reduce waste at all stages of construction. however, the importance of these construction wastes in terms of types and...

comin the case of pharmaceutical processes, the presence of preservatives can be problematic and the quantity is subject to stringent standards. so, the aim of this study is to quantify the removal of glycerin contained in ultrafiltration flat sheet membranes by filtration and soaking. this is carried out over a wide range of membranes with different characteristics. the selected flat sheet mem...

AIMS To assess which pathological features are associated with a sensitive marker of liver fibrogenesis and thus of the potential development of fibrosis in hepatitis C. METHODS The degree of liver fibrogenesis was evaluated by quantification of type I collagen mRNA and transforming growth factor (TGF) beta 1 mRNA (a major profibrogenic cytokine) in liver biopsy specimens from 28 patients wit...

BACKGROUND Maspin expression is a potential prognostic factor for various malignancies but its relation with gallbladder cancer is unknown and needs to be investigated needs to be investigated. We therefore here focused on maspin mRNA expression in normal, gall stone disease and gallbladder cancer subjects, with particular attention to prognostic importance in individuals with malignancies. M...