foot-and-mouth disease (fmd) is a severely contagious viral disease that mainly affects cloven-hoofedlivestock and wildlife. this study quantifies the cytokines mrna expression of vaccinated guinea pigs withfmd type o inactivated vaccine. blood samples were collected from eight guinea pigs at 7 and 28 days after thefirst vaccination. extracted mrnas were reverse-transcribed into cdna and analyz...

the mrna expression of growth hormone (gh) gene in early development stages of siberian sturgeon was investigated using rt-pcr method. samples were collected from unfertilized eggs up to 50 days post hatched (dph) larvae in 11 different times. ribosomal protein l6 (rpl6) transcripts were used as the internal standard during quantification of gh mrna expression. the results showed that the gh mr...

Objective(s): We aimed to examine association of gene expression of MOR1 and GluN1 at mRNA level in the lumbosacral cord and midbrain with morphine tolerance in male Wistar rats. Materials and Methods: Analgesic effects of morphine administrated intraperitoneally at doses of 0.1, 1, 5 and 10 mg/kg were examined using a hot plate test in rats with and without a history of 15 days morphine (10 mg...

Background: Adipose tissue is a main source for isolation of equine mesenchymal stem cells (MSCs) at different ages. It seems that characteristics of adipose-derived MSCs especially gene expression profile are changing along with age increase. A proper reference gene is required for normalizing data in gene expression analysis by qRT-PCR. This study aimed to evaluate whether GAPDH has a stable ...

Introduction: SOX9 is a transcriptional activator which is necessary for chondrogenesis. SOX6 are closely related to DNA-binding proteins that critically enhance its function. Therefore, to carry out the growth plate chondrocyte differentiation program, SOX9 and SOX6 collaborate genomewide. Chondrocyte differentiation is also known to be promoted by glucocorticoids through unknown molecular mec...

background : interleukin (il)-4 is a member of t helper 2 (th2) axis produced by t-lymphocyte and mast cell. it has been shown that il-4 expression changes in tumor tissues. the main objective of this study is to investigate the expression of il-4 mrna in patients with laryngeal squamous cell carcinoma (lscc) with or without lymph node involvement. materials and methods: mrna expression of il-4...

background: granuloca cells have a key role during estroeidogenesis. objectives: the purpose of this study was to determine the effect of number of culture medium granulosa cells on estradiol concentrations and mrna codding estrogenic and progestagenic enzyme. methods: briefly, follicles between 2 and 5 mm diameter were dissected from the ovaries of adult cows and were collected by rinsing the ...

objective(s): we aimed to examine association of gene expression of mor1 and glun1 at mrna level in the lumbosacral cord and midbrain with morphine tolerance in male wistar rats. materials and methods: analgesic effects of morphine administrated intraperitoneally at doses of 0.1, 1, 5 and 10 mg/kg were examined using a hot plate test in rats with and without a history of 15 days morphine (10 mg...