Background: It is demonstrated that c-Kit( receptor of stem cell factor) mRNA is expressed in late 2-cell stage to the expanded and hatched blastocyst and the stem cell factor (SCF) transcript is detected in the oviduct and uterus. The aim of this study was to investigate the effect of different doses of SCF on mouse 2-cell embryo development in vitro. Materials and Methods: 4-6 weeks old femal...

BACKGROUND/AIMS MicroRNA-29b (miR29b) has been previously identified in early mouse embryos through miRNA microarray analysis. Recent research has indicated that miR29b participates in DNA methylation by regulating DNA methyltransferase 3a/3b (Dnmt3a/3b) expression. However, the expression pattern and biological function of miR29b in mouse preimplantation embryonic development remain unknown. ...

Background: The efficiency of in vitro fertilization (IVF) is still low to be developed to blastocyst stage probably because of environmental conditions. It is likely that in vitro environment can not exactly mimic in vivo environment due to differences in media, metabolic content, atmospheric composition, temperature and pH. Therefore it may affect embryo quality by changing in embryo gene exp...

ABSTRACT Zygotic genomic activation (ZGA) is a landmark event in the maternal-to-zygotic transition (MZT), and regulation of ZGA by maternal factors remains to be elucidated. In this study, depletion ring finger protein 114 (RNF114), ubiquitin E3 ligase, led developmental arrest two-cell mouse embryos. Using immunofluorescence transcriptome analysis, RNF114 was proven play crucial role major ZG...

introduction: to improve the development of embryo in vitro several attempts have been made, including the application of leukemia inhibitory factor (lif). this factor plays an important role in embryo development and has been shown to enhance in vitro development rate in sheep and mice. there is controversy regarding the effect of lif on the different stages of embryo development. the aim of t...