L-methioninase degrades methionine, which is essential in methionine-dependent cancer cells, resulting specific cell death. Normal cells can synthesize their own methionine amino acids even the absence of exogenous methionine. This selective targeting makes a promising therapeutic candidate for cancer. In this study, was partially purified from Brevibacterium linens BL2. The activity enzyme fou...

introduction: embryonic stem (es) cells are pluripotent cells derived from the inner cell mass of blastocysts. these cells are appropriate for creation of animal models of human genetic diseases, the study of gene functions in vivo and differentiation into specific types as potential therapeutic agents for several diseases. we describe here production of transgenic chimeric mouse blastocysts by...

The ability of embryonic germinal cells (EG) to differentiate into primordial germinal cells (PGCs) and later into gametes during early developmental stages is a perfect model to address our hypothesis about cancer and infertility. This protocol shows how to isolate primordial germinal cells from developing gonads in 10.5-11.5 days post coitum (dpc) mouse embryos. Developing gonadal ridges from...

Sprouty negatively modulates branching morphogenesis in the Drosophila tracheal system. To address the role of mammalian Sprouty homologues in angiogenesis, another form of branching morphogenesis, a recombinant adenovirus engineered to express murine Sprouty-4 selectively in endothelial cells, was injected into the sinus venosus of embryonic day 9.0 cultured mouse embryos. Sprouty-4 expression...

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 208 No. 1 109–123 www.jcb.org/cgi/doi/10.1083/jcb.201404050 JCB 109 Correspondence to Mariusz Karbowski: [email protected] Abbreviations used in this paper: AvDev, average deviation; IMM, inner mitochondrial membrane; MEF, mouse embryonic fibroblast; Mff, mitochondrial fission factor; mito-PAGFP, mitochondrial matrix–targeted pho...

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 207 No. 5 643–656 www.jcb.org/cgi/doi/10.1083/jcb.201404128 JCB 643 Correspondence to Tina Izard: izard{at}scripps.edu Abbreviations used in the paper: CC, correlation coefficient; FA, focal adhesion; MEF, mouse embryonic fibroblast; PC, phosphatidylcholine; PIP2, phosphatidylinositol 4,5-bisphosphate; VBS, vinculin binding site; VH, vi...

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 204 No. 5 729–745 www.jcb.org/cgi/doi/10.1083/jcb.201306149 JCB 729 T. Mikawa and T. Maruyama contributed equally to this paper. Correspondence to Hiroshi Kondoh: [email protected] Abbreviations used in this paper: DKO, double knockout; MEF, mouse embryonic fibroblast; PGAM, phosphoglycerate mutase; SA--Gal, senescence-associa...

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 208 No. 4 443–455 www.jcb.org/cgi/doi/10.1083/jcb.201406102 JCB 443 Correspondence to Jason M. Haugh: [email protected] Abbreviations used in this paper: FMI, forward migration index; FP, fluorescent protein; GPI, glycophosphatidylinositol; MEF, mouse embryonic fibroblast; PDMS, polymethylsiloxane; PI3K, phosphoinositide 3-kinase; PI...

The purpose of this study was the surface modification of nano MOF-5 (NMOF-5) or IRMOF-1 (Zn4O(C8H4O4)3) in order to prevent its rapid degradation in the phosphate-buffered saline (PBS), along with the simultaneous increase in its biocompatibility. The NMOF-5 sample was synthesized under the ultrasound irradiation and then it was loaded wit...

The successful targeting of tumors can be achieved by conjugating moieties to nanoparticles. These modifications allow nannocarriers achieve greater specificity through binding specific receptors overexpressed on the surface tumor cells. In this study, pegylated liposomes encapsulating model drug/dye calcein and conjugated hyaluronic acid (HA) molecules were successfully synthesized, their abil...