The aim of the study was to standardize the pellet cryopreservation procedure for chicken semen. Mericanel della Brianza male chicken breeders (Italian breed) were used. Pooled semen samples were processed according to the following conditions: (1) dilution in prefreezing extender to 1 versus 1.5 bill cells/mL sperm working concentration (SWC); (2) 6% versus 9% dimethyl acetamide (DMA) concentr...

5.3 million American couples of reproductive age (9%) are affected by infertility, among which male factors account for up to 50% of cases, which necessitates the identification of parameters defining sperm quality, including sperm count and motility. In vitro fertilization (IVF) with or without intra cytoplasmic sperm injection (ICSI) has become the most widely used assisted reproductive techn...

INTRODUCTION Congenital bilateral absence of vas deferens (CBAVD) is a non-treatable cause of obstructive azoospermia (OA). However, the affected men can father children by undergoing sperm retrieval (SR) and intracytoplasmic sperm injection (ICSI). MATERIALS AND METHODS This video describes percutaneous epididymal sperm aspiration (PESA), performed on a 36 year--old male with CBAVD. In PESA ...

BACKGROUND Intrauterine insemination (IUI) is a valid treatment for infertility with a cumulative pregnancy rate of >40-90% after 3-10 treatment cycles. We studied the efficacy of a simplified method for motile sperm preparation for IUI. METHODS A prospective clinical trial was performed with 100 couples (male age 33-48 and female 28-37 years) with a 2-8 year history of primary infertility as...

I n the recent literature, several interesting articles have been published using the zona pellucdia (ZP)-bound sperm for intracytoplasmic sperm injection (ICSI) to enhance embryo quality, implantation and clinical pregnancy rates. The three studies were performed independently in different ART clinics. In the test group, patients’ sibling immature (either germinal vesicle (GV) or metaphase I) ...

UNLABELLED Pomegranate fruit (Punica granatum) and galangal (Alpinia galanga) have separately been shown to stimulate spermatogenesis and to increase sperm counts and motility in rodents. Within traditional medicine, pomegranate fruit has long been used to increase fertility, however studies on the effect on spermatogenesis in humans have never been published. With this study we investigated wh...

The hypothesis was tested that frozen bovine oviduct epithelial cells (BOEC) could provide a physiologic and standardized system for studying motility and acrosomal changes of fresh and frozen-thawed sperm. The BOEC were collected by lavage from cows and monolayers prepared from both fresh and frozen-thawed cells. Fresh and frozen semen processed from five bulls was extended in a modified Tyrod...

sting by E Abstract Introduction: Intrauterine insemination (IUI) is a valid treatment for infertility with a cumulative pregnancy rate of 40–90% after 3–10 treatment cycles. Design: We prospectively studied the efficacy of a new simplified method for motile sperm preparation for IUI for both male causes of infertility, and for those diagnosed with idiopathic infertility. Methods: A prospective...

OBJECTIVE To determine the frequency of and factors associated with delayed vasectomy success in men with first postvasectomy semen analysis showing motile sperm. DESIGN Descriptive study. SETTING One hospital-based family planning clinic and two private clinics from the Quebec City area, Canada. PATIENT(S) Three hundred nine men vasectomized between 1990 and 2001 and who had a first seme...

The mechanisms responsible for mediating the influence of sperm preparation protocols on human sperm function have been investigated. Techniques that involved the separation of motile spermatozoa prior to centrifugation were found to yield sperm suspensions of highest quality. If the spermatozoa were centrifuged prior to isolation of the motile cells, sperm function was impaired. The detrimenta...