1. Methods for the use of the marine green alga, Ulva lactuca, in studies on electrolyte metabolism are described. 2. The effect of illumination and iodoacetate on the potassium and sodium content, as well as the influence of light and running sea water on the iodoacetate effect was investigated. The rate of exchange of cellular potassium ion for K(42) under conditions of light and dark at 20 a...

Measurements of lactate production and ATP concentration in superfused rat retinas were compared with extracellular photoreceptor potentials (Fast PIII). The effect of glucose concentration, oxygen tension, metabolic inhibition, and light were studied. Optimal conditions were achieved with 5-20 mM glucose and oxygen. The isolated retina had a high rate of lactate production and maintained the A...

lodoacetic acid and its amide have been widely used in protein chemistry as reagents for sulfhydryl groups. Although both can be expected to undergo other reactions with proteins, for example, with the e-amino groups of lysine residues, the imidazole groups of histidine residues, and the phenolic hydroxyls of tyrosine residues, these reactions arc generally slower than is the one with sulfhydry...

Introduction: Although, the effect of direct intra-articular injection of bone marrow stem cells (BMSCs) on the repair of articular cartilage and the effect of Elaeagnus angustifolia extract on pain relief in patients with osteoarthritis have been investigated, no studies has been conducted to compare the effects of these two therapeutic methods on the mechanical properties of articular cartila...

The reaction of iodoacetic acid with ribonuclease has been studied recently by Gundlach et al. (1). Inactivation of the enzyme occurred over a wide range of pH. The authors concluded that at acid pH the reaction involved solely methionyl residues, that at pH 5.5 a histidyl residue was the principal site of attack, and that at alkaline pH values significant reaction at lysyl residues occurred. O...

The pH-rate profile for inactivation of the RTEM-1 cysteine beta-lactamase by iodoacetate supports previous evidence [Knap & Pratt (1989) Proteins Struct. Funct. Genet. 6, 316-323] for the activation of the active-site thiol group by adjacent functional groups. The enhanced reactivity of iodoacetate, with respect to that of iodoacetamide, suggests the influence of a positive charge in the activ...

The production of endogenous pyrogen by intact granulocytes obtained from acute peritoneal exudates is blocked by arsenite, iodoacetate, p-chloromercuribenzoate, and N-ethylmaleimide in concentrations of 2 x 10(-4)M. When the concentration of these sulfhydryl-reactive enzyme inhibitors is increased to 2 x 10(-2)M, only the iodoacetate inactivates the pyrogen molecule, whereas the arsenite, the ...

Desktop analyzers and single-test meters used in primary healthcare are mainly calibrated to measure whole blood. To minimize matrix effects of control materials in external quality assessment schemes, we stabilized fresh human EDTA-treated blood with sodium iodoacetate (1.8 mmol/L). The whole-blood based control material was useful for control of hemoglobin and glucose, the two most common ana...

Of several preservatives we tested, a mixture of "isotonic" sodium fluoride and sodium iodoacetate (17.7 and 5.0 g/L, respectively) best inhibits glucose loss from blood collected from the newborn. However, the inhibition is incomplete. Twenty microliters of blood is diluted with 100 microliter of preserving solution. After centrifugation, the glucose in 100 microliter of prewarmed supernate (d...