BACKGROUND Cytokines secreted by adipose tissue macrophages (ATMs) significantly alter adipocyte function, inducing inflammatory responses and decreasing insulin sensitivity. However, little relevant information is available regarding the role of microvesicles (MVs) derived from ATMs in macrophage-adipocyte crosstalk. METHODS MVs were generated by stimulation of M1 or M2 phenotype THP-1 macro...

Gram-negative bacteria produce extracellular outer membrane vesicles (OMVs) that interact with host cells. Unlike Gram-negative bacteria, less is known about the production and role of extracellular membrane vesicles (MVs) in Gram-positive bacteria. The food-borne pathogen Listeria monocytogenes can survive under extreme environmental and energy stress conditions and the transcription factor σ(...

SAS@offers the option of using hiperspaces as the standard WORK file under MVS/ESA” as a performance en.tiancement. This paper introduces hiperspaces under MVS, and the applicable SAS options to invoke hiperspace use. Also, attention is given to tuning the use of hiperspaces within MVS (i.e. MVS’5 SRM parameters). Performance gains can be astonishing with little or no work on the part of the us...

Exosomes/microvesicles (MVs) provide a mechanism of intercellular communication. Our hypothesis was that mesenchymal stromal cells (MSC) from myelodysplastic syndrome (MDS) patients could modify CD34+ cells properties by MVs. They were isolated from MSC from MDS patients and healthy donors (HD). MVs from 30 low-risk MDS patients and 27 HD were purified by ExoQuick-TC™ or ultracentrifugation and...

There is a rapidly growing body of evidence that production of microvesicles (MVs) is a universal feature of cellular life. MVs can incorporate microRNA (miRNA), mRNA, mtDNA, DNA and retrotransposons, camouflage viruses/viral components from immune surveillance, and transfer cargo between cells. These properties make MVs an essential player in intercellular communication. Increasing evidence su...

When designing a cleanroom to achieve a required airborne cleanliness class or grade, such as specified in the International Organization for Standardization (ISO) 14644-11, or the European Union Guidelines to Good Manufacturing Practice (EU GGMP)2, designers have a problem in deciding how much filtered air should be supplied to the cleanroom. Currently, this is not normally calculated but base...

The indoor air quality in an operating theatre affects both patient health and the well-being of the surgical team members. Health care facilities, and operating theatres in particular, require ventilation and air conditioning for thermal comfort and removal of contaminants. Although the patient undergoing surgery is given the highest priority, it is important to consider the comfort of surgica...

Membrane-derived microvesicles (MVs) are released from the cell surface and are implicated in cell-to-cell communication. We evaluated whether MVs derived from endothelial progenitor cells (EPCs) are able to trigger angiogenesis. We found that EPC-derived MVs were incorporated in endothelial cells by interaction with 4 and 1 integrins expressed on the MV surface. In vitro, MVs promoted endothel...

A derecho-producing bow-echo event over the central United States on 8 May 2009 is analyzed based on radar observations and a successful real-data WRF simulation at 0.8-km grid spacing. Emphasis is placed on documenting the existence, evolution, and characteristics of low-level mesovortices (MVs) that form along the leading edge of the bowing system. The genesis of near-surface high winds withi...

The ectonucleoside pyrophosphatase phosphodiesterase 1 (NPP1/PC-1) is a member of the NPP enzyme family that is critical in regulating mineralization. In certain mineralizing sites of bone and cartilage, membrane-limited vesicles [matrix vesicles (MVs)] provide a sheltered internal environment for nucleation of calcium-containing crystals, including hydroxyapatite. MV formation occurs by buddin...