Here, we present a low-cost method to produce compact arrays using microporous materials and reagent jetting. Oligonucleotides are immobilized on membrane sheets as a series of lines. The membrane sheet is then rolled and bound, and the spiral structure is cut like a "jelly roll" to produce identical arrays. The spiral arrays behave much like larger formats using membranes, and hybridization de...

This technical note presents a fabrication method and applications of three-dimensional (3D) interconnected microporous poly(dimethylsiloxane) (PDMS) microfluidic devices. Based on soft lithography, the microporous PDMS microfluidic devices were fabricated by molding a mixture of PDMS pre-polymer and sugar particles in a microstructured mold. After curing and demolding, the sugar particles were...

There is little information showing the use of microporous polypropylene hollow fiber oxygenators during extra-corporeal life support (ECLS). Recent surveys have shown increasing use of these hollow fibers amongst ECLS centers in the United States. We performed a retrospective analysis comparing the Terumo BabyRx hollow fiber oxygenator to the Medtronic 800 silicone membrane oxygenator on 14 ne...

Uniformly sized microparticles of poly(D,L-lactic-co-glycolic) (PLGA) acid, with controllable median diameters within the size range 40-140 microm, were successfully prepared by membrane emulsification of an oil phase injected into an aqueous phase, followed by solvent removal. Initially, simple particles were produced as an oil in water emulsion, where dichloromethane (DCM) and PLGA were the o...

One of the main functions of the airway mucosa is to maintain a mechanical barrier at the air-surface interface and to protect the respiratory tract from external injuries. Differentiation of human airway epithelial cells (hAECs) to polarized airway mucosa can be reproduced in vitro by culturing the cells on microporous membrane at the air-liquid interface. Here, we describe approaches to study...

Simultaneous separation of vapor and particles in industrial processes could be a key step toward manufacturing of high-quality goods. The separation is critical for successful measurement of volatile or semi-volatile aerosol particles, which no reliable technique exists. We have developed a technique for separation of vapor and particles simultaneously using a specialty microporous metallic me...

Gaseous microemboli (GME) are still an unsolved problem of extracorporeal circuits. They are associated with organ injury during cardiopulmonary bypass. Microbubbles of different sizes and number are generated in the blood as the result of different components of the extracorporeal circuit as well as surgical maneuvers. The aim of our study was to observe the behavior of microporous membrane ox...

The Boyden chamber assay, originally introduced by Boyden for the analysis of leukocyte chemotaxis, is based on a chamber of two medium-filled compartments separated by a microporous membrane. In general, cells are placed in the upper compartment and are allowed to migrate through the pores of the membrane into the lower compartment, in which chemotactic agents are present. After an appropriate...

Microporous carbon catalysts with large surface areas (800-1100 m(2) g(-1)) and high densities of SO3H groups (ca. 1.1 mmol g(-1)) were synthesized by sulfonation of zeolite-templated microporous carbon. The resulting SO3H-bearing microporous carbon catalysts exhibited higher catalytic performance for the hydrolysis of cellobiose and the Beckmann rearrangement than conventional solid acid catal...