PURPOSE The present study was an attempt to determine the spontaneous frequency of micronuclei (MN) in newborns from High Level Natural Radiation Areas (HLNRA) and the adjoining Normal Level Radiation Areas (NLNRA) of the monazite-bearing Kerala Coast in Southern West India using Cytochalasin Blocked Micronuclei (CBMN) assay. MATERIALS AND METHODS Human umbilical cord blood samples were colle...

Benzene is a widely recognized human and animal carcinogen. In spite of considerable research, relatively little is known about the genotoxic events that accompany benzene exposure in vivo. To gain insights into the mechanisms underlying the genotoxic effects of benzene, we have characterized the origin of the micronuclei that are formed in bone marrow erythrocytes and splenic lymphocytes of be...

Cytochalasine B is an indicator in the CBMN assay, but can also be geno- or cytotoxic. The aim of study was to determine optimal dose cytochalasine for Cytokinesis-Block Micronucleus (CBMN) assay domestic cat. results revealed a surprisingly high number micronuclei (MN) relation doses used. It found that concentration 2.5 μg/ml enough obtain binuclear cells, with minimal toxic effect on lymphoc...

Background We intend to study the inhibitory effect of sulfur compound in Ramsar hot spring mineral on tumor-genesis ability of high natural background radiation. Objective The radioprotective effect of sulfur compounds was previously shown on radiation-induced chromosomal aberration, micronuclei in mouse bone marrow cells and human peripheral lymphocyte. Ramsar is known for having the highes...

Micronucleus (MN) is a biomarker widely used in biomonitoring studies carried out to determine the genetic risk associated to pesticide exposure. Many in vitro and in vivo studies, as well as epidemiological approaches, have demonstrated the ability of certain chemical pesticides to produce genetic effects including cancer and other chronic pathologies in humans; thus, biomonitoring studies hav...

INTRODUCTION Methotrexate, a folate antagonist, is a mainstay treatment for childhood acute lymphoblastic leukemia. It is also widely used in a low dose formulation to treat patients with rheumatoid arthritis. In rats, methotrexate is known to induce micronuclei formation, leading to genetic damage, while vitamin A is known to protect against such methotrexate-induced genetic damage. Leucovorin...