gaining acceptance in pharmacological studies (1,2). Numerous studies have demonstrated the capability of microdialysis sampling in characterizing the behavior of exogenous compounds in tissues such as blood (3-6), muscle (7,8), solid tumor (9), adipose (7,10), liver (1113), and dermis (14-17). Of all the commercially available probe designs, the BAS linear tissue probe (F1) is especially suita...

Excessive production of reactive oxygen species (ROS) are implicated in the pathogenesis of numerous disease states. However, direct measurement of in vivo ROS in humans has remained elusive due to limited access to appropriate tissue beds and the inherently short half-lives and high reactivity of ROS. Herein, we describe a novel technique by which to measure in vivo ROS in human skeletal muscl...

The authors are grateful to Dr. Aygul Balcioglu, Research Scientist at MIT for providing fresh microdialysis samples for these experiments. Overview Purpose: To develop an Ultra High Performance Liquid Chromatography (UHPLC) method to resolve the major biogenic amine and their acid metabolites in a microdialysis sample collected from the rat brain with improved throughput using the Thermo Scien...

Cutaneous microdialysis is a method of measuring endogenous and exogenous compounds in the dermal interstitial fluid. The microdialysis probe is inserted in the dermis using a guide cannula. The insertion trauma was studied in dorsal forearm skin in a total of 28 human healthy volunteers. Twenty-four volunteers received local anaesthesia (Xylocain 10 mg/ml) in both forearms and a microdialysis ...

OBJECTIVE We examined the reliability of two continuous glucose sensors in type 1 diabetic patients at night and during rapid glucose excursions and verified the hypothesized nocturnal hypoglycemic drift of the needle-type sensor (CGMSgold) and delay of the microdialysis sensor (GlucoDay). RESEARCH DESIGN AND METHODS Blood was sampled overnight twice per hour in 13 patients. Rapid-acting insu...

Mikkelsen UR, Helmark IC, Kjær M, Langberg H. Prostaglandin synthesis can be inhibited locally by infusion of NSAIDS through microdialysis catheters in human skeletal muscle. J Appl Physiol 104: 534–537, 2008. First published December 13, 2007; doi:10.1152/japplphysiol.01016.2007.—Prostaglandins are known to be involved in the regulation of local blood flow within human skeletal muscles during ...

INTRODUCTION Tight glucose control in the ICU has been proven difficult with an increased risk for hypoglycaemic episodes. Also the variability of glucose may have an impact on morbidity. An accurate and feasible on-line/continuous measurement is therefore desired. In this study a central vein catheter with a microdialysis membrane in combination with an on-line analyzer for continuous monitori...

Thyrotoxicosis increases endogenous glucose production (EGP) and induces hepatic insulin resistance. We have recently shown that these alterations can be modulated by selective hepatic sympathetic and parasympathetic denervation, pointing to neurally mediated effects of thyroid hormone on glucose metabolism. Here, we investigated the effects of central triiodothyronine (T(3)) administration on ...

BACKGROUND Cerebral cytoplasmatic redox state is a sensitive indicator of cerebral oxidative metabolism and is conventionally evaluated from the extracellular lactate/pyruvate (LP) ratio. In the present experimental study of global cerebral ischemia induced by hemorrhagic shock, we investigate whether the LP ratio obtained from microdialysis of cerebral venous blood may be used as a surrogate m...