The chip and quantitative real-time PCR (qPCR) assays were optimized to study the expression of microcystin biosynthesis genes (mcy) with RNA samples extracted from cyanobacterial strains and environmental water samples. Both microcystin-producing Anabaena and Microcystis were identified in Lake Tuusulanjärvi samples. Microcystis transcribed the mcyE genes throughout the summer of 2006, while e...

Water samples were collected during 3 years (2004-2007) at three sampling sites in the Rio de la Plata estuary. Thirteen biological, physical, and chemical parameters were determined on the water samples. The presence of microcystin-LR in the reservoir samples, and also in domestic water samples, was confirmed and quantified. Microcystin-LR concentration ranged between 0.02 and 8.6 μg.L(-1). Pr...

Recently, there has been a rise in freshwater harmful algal blooms (HABs) globally, as well as increasing aquaculture practices. HABs can produce cyanotoxins, many of which are hepatotoxins. An ultra-performance liquid chromatography tandem mass spectrometry method was developed and validated for nine cyanotoxins across three classes including six microcystins, nodularin, cylindrospermopsin and...

The worldwide occurrence of cyanobacterial blooms due to water eutrophication evokes extreme concerns. These blooms produce cyanotoxins which are hazardous to living organisms. So far among these toxins, Microcystin-LR (MC-LR) is the most toxic and the most frequently encountered toxin produced by the cyanobacteria in the contaminated aquatic environment. Microcystin-LR is a potential carcinoge...

"Super-blooms" of cyanobacteria that produce potent and environmentally persistent biotoxins (microcystins) are an emerging global health issue in freshwater habitats. Monitoring of the marine environment for secondary impacts has been minimal, although microcystin-contaminated freshwater is known to be entering marine ecosystems. Here we confirm deaths of marine mammals from microcystin intoxi...

Microcystin is a toxin secreted by cyanobacteria, which are the dominant phytoplankton in eutrophic freshwater bodies. The present study reports histopathological, hematological and biochemical changes in the freshwater fish, Heteropneustes fossilis due to toxicity of microcystin. Toxic effects were observed in liver, kidney, corpuscles of Stannius, gills and gonads. Bilirubin concentration inc...

An 8-year-old Holsteiner gelding was presented for evaluation of anorexia, obtundation, icterus, and mild colic signs of 48 hours duration. History, physical examination, and initial diagnostics were suggestive of hepatic disease and encephalopathy. Microcystin toxicosis was suspected based on historical administration of a cyanobacteria supplement, associated serum biochemistry abnormalities, ...

Abstract The adverse effects of exposure to microcystins in terrestrial crops have been well documented. However, the retention and bioavailability microcystin-LR, one most prevalent cyanotoxins, from soil plants, is poorly understood. In present study, amount free microcystin-LR two types, a silty sand clayey loam, with three toxin concentrations time was investigated. Using on Medicago sativa...

Abstract Many populations of water fleas ( Daphnia ) are exposed to algal blooms dominated by microcystin‐producing cyanobacteria. However, the severity these effects on fitness remain poorly understood in natural populations. We investigated seasonal changes body size, reproduction and survival D . longispina individuals from five eutrophic lakes southern Sweden. tested whether collected befor...

Microcystin-LR and -LA were readily biodegraded by a bacterium, Sphingpoyxis sp. LH21, in a treated reservoir water. Detection of the microcystins was conducted using high-performance liquid chromatography (HPLC), protein phosphatase 2A (PP2A) inhibition assay and a cell-based cytotoxicity assay. The HPLC results correlated well with the two assays. The decrease in cytotoxicity, coupled with th...