Protein phosphatase C was purified 1500-fold from rat liver by a six-step procedure including a fractionation step with 80% ethanol at room temperature and two successive chromatographic separations on DEAESephadex. This preparation restored hydrox~ethyl glutaryl-CoA (HMG-CoA) reductase activity in liver microsomes pretreated with MgATP and also inactivated HMG-CoA reductase kinase. The relativ...

A monomeric (Mr = 43,000) NADPH-dependent oxidoreductase with a broad substrate specificity for aliphatic and aromatic aldehydes and aldo sugars has been purified from the skeletal muscle of female and castrated pigs. The properties of this enzyme are consistent with those of aldose reductase (EC 1.1.1.21), and the enzyme is immunologically identical with aldose reductase from pig lens. However...

Atherosclerotic plaque regression and HMG-CoA reductase inhibition potential of curcumin: An integrative omics in-vivo studyPriyanka Riyad, Ashok Purohit, Karishma Sen, Heera Ram

3-Hydroxy-3-methylglutaryl CoA reductase (HMG-CoA reductase) plays a rate-limiting role in isoprenoid biosynthesis and is associated with cell proliferation and transformation. Although an elevated level of HMG-CoA reductase activity is consistently detected in cancer cell lines and tumors, the question remains whether HMG-CoA reductase activity may have a causative role in cell transformation....

PURPOSE Increased flux of glucose via the polyol pathway, oxidative stress and ischaemia lead to the upregulation of the aldose reductase (AR), the key enzyme of the polyol pathway. This adversely affects the organism and can in part be reduced by inhibition of the enzyme. METHODS In this study, we examined the effect of the HMG-CoA-reductase inhibitor atorvastatin on the expression of aldose...

The activity of NADH-cytochrome b5 reductase (NADH-methemoglobin reductase) is generally reduced in red cells of patients with recessive hereditary methemoglobinemia. To determine whether this lower activity is due to reduced concentration of an enzyme with normal catalytic properties or to reduced activity of an enzyme present at normal concentration, we measured erythrocyte reductase concentr...

Aldose reductase, a member of the aldo-keto reductase family, has been implicated in the development of vascular and neurological complications in diabetes. Despite recent studies from our laboratory demonstrating protection of ischemic hearts by an aldose reductase inhibitor, the presence and influence of aldose reductase in cardiac tissue remain unknown. Our goal in this study was to isolate ...