background: transcriptional silencing of tumor suppressor genes and tumor related genes like gstp1 by methylation of promotor region cpg island is believed to be an important mechanism in tumorigenesis. the gstp1 gene encodes the enzyme glutathione s-transferase pi which defends the cells against oxidative damage and electrophilic carcinogens. methods: to gain insight into the role of epigeneti...

background: stem cell differentiation is a process composed of vast variety of factors which are controlled by a network of certain mechanisms. this study aims to determine the possible role of dna methylation, a potent regulator of vhl, ecad and runx3 genes during erythroid differentiation driven by mir-451. materials and methods: to determine the methylation status of promoters and the expres...

the aim of this study was to analyze the polymorphisms existing in the 5´ flanking region, exonic, and some parts of the intronic regions as well as methylation analysis of the ednrb gene in cattle and goat. these regions were sequenced in three different breeds of cattle including sistani, golpayegani, and holstein and were compared with that of marghoz goat. the results identified that this g...

objectives: myelodysplastic syndromes (mdss) include a diverse group of clonal bone marrow disorders characterized by ineffective hematopoiesis and pancytopenia. it was found that down regulation of apaf1 , a putative tumor suppressor gene, leads to resistance to chemotherapy and disease development in some cancers. in this study, we investigated the relation of apaf1 methylation status with it...

conclusions the polymorphism and promoter methylation of rassf1a may be a significant factor in hcc, and can be an indicator for poor prognosis in patients with hcc. background the functional and prognostic significance of ras association domain family 1a gene (rassf1a) on hepatocellular carcinoma (hcc) has not been well characterized. objectives this study aimed to investigate the association ...

Background: The protein of Niemann-pick type C1 (NPC1) gene promotes the egress of cholesterol from late endosomes and lysosomes to other cellular compartments and contributes to a process known as reverse cholesterol transport. This study aimed to examine whether promoter methylation of NPC1 is associated with risk of cardiovascular disease (CVD). Methods: Fifty CVD patients and 50 healthy sub...

Background: Aberrant methylation of cytosine-guanine dinucleotide islands leads to inactivation of tumor suppressor genes in breast cancer. Tumor suppressor genes are unmethylated in normal tissue and often become hypermethylated during tumor formation, leading to gene silencing. We investigated the association between E-cadherin (CDH1) and estrogen receptor-α (ESRα) gene promoter methylation a...

DNA methylation as an epigenetic mediator plays the important role in spatial and temporal gene regulation and ensures the stability and the plasticity of organism. In this investigation, methylation sensitive amplification polymorphism (MSAP) were assessed in CCGG sites on a halophytic plant, Aeluropuslittoralis in response to different temperature stresses including freezing...