Background : Gestational trophoblastic neoplasia (GTN) refers to malignant lesions that arise from abnormal proliferation of placental trophoblast. Even in its metastatic forms GTN is curable with a cure rate of 90-100 %. Currently, methotrexate with or without folic acid, andactinomycin D is recommended for low risk GTN. The aim of this study is to compare the efficacy of methotrexate and ac...

Coadministration of methotrexate and nonsteroidal anti-inflammatory drugs (NSAIDs) can cause a pharmacokinetic interaction and a subsequent increase in blood methotrexate concentrations. methotrexate and most NSAIDs are excreted into urine via organic anion transporter 3 (OAT3). The purpose of this study was to evaluate NSAIDs that compete less with methotrexate by using the renal cell line sta...

Transport of methotrexate (4amino-lo-methylpteroylglutamic acid), a structural analogue of folic acid, was studied in L1210 mouse leukemia cells grown in tissue culture. Intracellular methotrexate was present in two states. (a) There was an essentially nonexchangeable intracellular fraction probably bound to the enzyme dihydrofolate reductase. (b) The remaining intracellular methotrexate was ra...

Ten specific examples of the underestimation of the efficacy, effectiveness and tolerability, and overestimation of adverse events of weekly, low-dose methotrexate, administered with folic acid, in treatment of rheumatic diseases are summarised. These examples include: 1) meta-analyses of clinical trials suggest that methotrexate has an efficacy similar to other disease-modifying anti-rheumatic...

We have carried out a clinical trial in 23 patients to determine whether dipyridamole modulates the clinical effect of methotrexate. This trial was based upon in vitro studies which indicate that dipyridamole potentiates the cytotoxic action of methotrexate through inhibition of thymidine salvage. Methotrexate was given as a bolus injection 24 h after initiation of a high dose dipyridamole infu...

BACKGROUND To review our experience using methotrexate as a single long-term immunosuppressant (IS) therapy in neuromyelitis optica/neuromyelitis optica spectrum disorders (NMO/NMOSD). METHODS We performed a retrospective chart review of all patients with a diagnosis of NMO/NMOSD, supported by a positive NMO-IgG testing, who were treated with methotrexate. A paired sample 2 tailed t test was ...

Influx of [3H]methotrexate into freshly isolated hepatocytes is mediated by highand low-affinity processes, both of which are inhibited by the bile salts, cholate, taurocholate, and de oxycholate, and the organic anions, bromosulfophthalein and rose bengal. At 100 @Mconcentrations, these compounds inhibit 1 @LM [3H]methotrexate influx by 70 to 90%. Dixon plots established a similar K (-@†̃20 @...

Influx of [3H]methotrexate into freshly isolated hepatocytes is mediated by highand low-affinity processes, both of which are inhibited by the bile salts, cholate, taurocholate, and de oxycholate, and the organic anions, bromosulfophthalein and rose bengal. At 100 @Mconcentrations, these compounds inhibit 1 @LM [3H]methotrexate influx by 70 to 90%. Dixon plots established a similar K (-@†̃20 @...

placenta increta, a rare complication of pregnancy, is associated with significant postpartum hemorrhage often requiring emergency hysterectomy. we report a case of conservative management, with a combination of parenteral methotrexate, serial ultrasound and β-hcg assessment. serum β-hcg levels were undetectable after 8 weeks of therapy. a scan at 6 months showed complete involution of the uter...