A high performance liquid chromatography method for determining the levels of 4-pregnene-3-ones in tissue containing fat, e.g. in thymus is reported. Following the extraction with chloroform/methanol the fat has been separated from the steroidal-fraction by using disposable extraction-columns. The steroidal-fraction has been analysed in two separation systems, on a RP18-column with a methanol/w...

BACKGROUND Prunus yedoensis (PY) is a traditional anti-allergy and anti-inflammatory herb medicine used in South Korea. However, until date, little is known regarding its mechanism of action. METHODS In order to elucidate the mechanism of anti-inflammatory effect of PY, the constituents of PY were analysed by high performance liquid chromatography (HPLC), and nitric oxide (NO) and prostagland...

BACKGROUND Myrsine africana is an herbaceous plant that is traditionally used as appetizer and carminative. Locally, it is used for the treatment of pulmonary tuberculosis, rheumatism and diarrhea by healers. The aims of the current study were to screen the crude methanol extract obtained from the aerial parts (leaves and stem) of M. africana, for antispasmodic actions on isolated tissues and f...

Monodisperse polystyrene nanoparticles with a diameter of 173 nm were incorporated into a polydimethylsiloxane matrix where they display an iridescent color that can be attributed to the photonic crystal effect. The film is of violet color if placed in plain water, but turns to red in the presence of the non-polar solvent n-hexane. Several solvents were studied in some detail. We show that such...

Despite a long tradition of use in the treatment of mental disorders, Viscum album L. (Ujjral; family – Viscaceae) has not been systematically investigated to validate its traditional claims. The methanol extract of V. album aerial parts, its ethyl acetate fraction (EAF) and 1-butanol fraction (BF) were screened for various neuropharmacological activities. The methanol extract, EAF and BF exhib...

background and objectives: alzheimer's disease (ad) is an age dependent disorder. ad is associated with decrease of brain acetylcholine level. nowadays, one of the methods for progression inhibition of ad is using acetylcholinesterase inhibitors. rosaceae is a large plant family. different biological effects of some species of this family have been reported. the aim of the present study was to ...

objectives lipid components of spermatozoa have an important role in its functional activity. in this study the concentration of cholesterol, phospholipid, triacylglycerol and total lipid in different subsets of human spermatozoa, isolated as three fractions by a discontinuous puresperm gradient, was determined. methods aliquots of the liquefied semen samples (n=107) were layered on top of the ...

The coadsorption of methanol (CH3OH) and water (D2O) on the MgO~100!/Mo~100! surface at 100 K has been studied by metastable impact electron spectroscopy ~MIES!, ultraviolet photoelectron spectroscopy @UPS ~HeI!#, and by thermal programmed desorption ~TPD!. Methanol wets the MgO surface and adsorbs nondissociatively within the first monolayer with the hydroxyl group oriented toward the substrat...

In this work, we prepared the nafion/montmorillonite/heteropolyacid nanocomposite membranes for direct methanol fuel cells (DMFCs). The analyses such as X-ray diffraction (XRD), Fourier transform infrared (FTIR), and scanning electron microscopy (SEM) were conducted to characterize the filler dispersion and membrane structure in prepared nanocomposite membranes. XRD patterns of nafion-CsPW-MMT ...

With the procedure which is described, brain tissue is separated into six fractions, the sum of whose weights equals the dry weight of the tissue. Three of these primary fractions are further separated into subfractions. Recovery is quantitative at each step. The procedure, as applied to rat brain, gave consistent results; duplicate analyses of the two halves of brains, divided as equally as po...