We describe the high resolution structure of the large ribosomal subunit from Deinococcus radiodurans (D50S), a gram-positive mesophile suitable for binding of antibiotics and functionally relevant ligands. The over-all structure of D50S is similar to that from the archae bacterium Haloarcula marismortui (H50S); however, a detailed comparison revealed significant differences, for example, in th...

The Actinobacteria, Corynebacterium pseudotuberculosis strain P54B96, a nonmotile, non-sporulating and a mesophile bacterium, was isolated from liver, lung and mediastinal lymph node lesions in an antelope from South Africa. This strain is interesting in the sense that it has been found together with non-tuberculous mycobacteria (NTMs) which could nevertheless play a role in the lesion formatio...

Mean macromolecular dynamics was quantified in vivo by neutron scattering in psychrophile, mesophile, thermophile and hyperthermophile bacteria. Root mean square atomic fluctuation amplitudes determining macromolecular flexibility were found to be similar for each organism at its physiological temperature ( approximately 1 A in the 0.1 ns timescale). Effective force constants determining the me...

Thermotropic properties of blue-green algae grown at high, room, and low temperatures in H(2)O and D(2)O media were studied by highly sensitive differential scanning microcalorimetry. The thermograms of these organisms contain an endothermal peak in the temperature range of 50 to 70 C with an endothermal heat ranging from 0.14 to 1.91 joules per gram organism. The temperature at which the endot...

Ribonucleases H from the thermophilic bacterium Thermus thermophilus and the mesophile Escherichia coli demonstrate a dramatic and surprising difference in their change in heat capacity upon unfolding (DeltaCp degrees ). The lower DeltaCp degrees of the thermophilic protein directly contributes to its higher thermal denaturation temperature (Tm). We propose that this DeltaCp degrees difference ...

The genes (mdh) encoding malate dehydrogenase (MDH) from the mesophile Chlorobium vibrioforme and the moderate thermophile C. tepidum were cloned and sequenced, and the complete amino acid sequences were deduced. When the region upstream of mdh was analyzed, a sequence with high homology to an operon encoding ribosomal proteins from Escherichia coli was found. Each mdh gene consists of a 930-bp...

background and objectives: in the era of global industrialization, enzymes are being used extensively in the various sectors including food processing. owing to the high price of enzymes, various initiatives have been undertaken by the r&d; sector for the development of new processes or improvement in the existing processes for production of cost effective enzymes. with the advancement in the f...

pharmaceutical products prepared in pharmacies have the potential of contamination with different microorganisms. this is in part due to the unhygienic environment and also lack of a suitable preservative system. in this study, microbial quality of eucerin-urea ointments prepared at different pharmacies in tehran, at the point of sale and also after two weeks storage at room temperature was exa...

The different combinations of bioactive compounds, probiotics (Streptococcus thermophilus ATCC 19258 and Lactobacillus bulgaricus BAA-2844), prebiotic (fructooligosaccharides, FOS), fenugreek, L-arginine, were added to the pâtė meat. Some meats contaminated with Salmonella Typhimurium 14028 Listeria monocytogenes 7644. Fermentation was applied only batches containing ‘probiotic’ or ‘probiotic p...