The solid-phase Edman degradation procedure as proposed by Laursen [ 1] is ideally suited to the evaluation of peptidyl-resin products resulting from Merrifield solid-phase synthesis [2,3] of peptides. Incomplete deprotection and incomplete coupling reactions which occur during synthesis have been detected using quantitative Edman degradation [4,5]. However, extended sequencing experiments were...

Ribonuclease A (RNase A; EC 3.1.27.5) was perhaps the most studied enzyme of the 20th century and is the best characterized ribonuclease. The “A” in its name refers not to its substrate specificity, but to the predominant form of the enzyme produced by the bovine pancreas. RNase A is unmodified, whereas RNase B, RNase C, and RNase D are mixtures of glycoforms. Because of its availability in lar...

Pancreatic ribonuclease A (EC 3.1.27.5, RNase) is, perhaps, the best-studied enzyme of the 20th century. It was isolated by René Dubos, crystallized by Moses Kunitz, sequenced by Stanford Moore and William Stein, and synthesized in the laboratory of Bruce Merrifield, all at the Rockefeller Institute/University. It has proven to be an excellent model system for many different types of experiment...

A new approach on the use of mass spectrometry direct-insertion and a quadrupole detector for analysis of organic compounds supported in solid phase has been developed. This is a simple and efficient method based on cleavage due to the thermal-instability of the benzylic group of most commercial resins. The cleavage of supported compounds takes place in the spectrometer as a consequence of the ...

Cephalometric evaluation of Class III malocclusion by a modified TweedMerrifield analysis Mayury Kuramae1 Maria Beatriz Borges de Araújo Magnani2 João Sarmento Pereira Neto2 Darcy Flávio Nouer3 Paulo Roberto Aranha Nouer4 1MDS, DDS Student, FOP/UNICAMP 2DDS, Professor of the Pediatric Clinic, FOP/ UNICAMP 3Chairman, Department of the Pediatric Clinic, FOP/UNICAMP 4Coordinator of the Postgraduat...

OBJECTIVE The aim of this study was to determine the standards of facial height in 30 young (14-year-old) Japanese-Brazilian descendants with normal occlusion, and assess whether sexual dimorphism is evident. METHODS The cephalometric measurements used followed the analyses by Wylie-Johnson, Siriwat-Jarabak, Gebeck, Merrifield and Horn. RESULTS Results showed dimorphism for total anterior f...

Denote by B∗ n the set of all k∗-cycle resonant hexagonal chains with n hexagons. For any Bn ∈ B ∗ n , let m(Bn) and i(Bn) be the numbers of matchings (=the Hosoya index) and the number of independent sets (=the Merrifield-Simmons index) of Bn, respectively. In this paper, we give a characterization of the k∗-cycle resonant hexagonal chains, and show that for any Bn ∈ B ∗ n , m(Hn) ≤ m(Bn) and ...