Pseudomonas putida produces two lipoamide dehydrogenases, LPD-glc and LPD-val. LPD-val is specifically required as the lipoamide dehydrogenase of branched-chain keto acid dehydrogenase and LPD-glc fulfills all other requirements for lipoamide dehydrogenase. Both proteins are dimers with one FAD per subunit. LPD-glc has an absorption maximum at 455 nm, but LPD-val has a maximum at 460 nm. Compar...

The conformational dynamics of wild-type Escherichia coli thioredoxin reductase (TrxR) and the mutant enzyme C138S were studied by ultrafast time-resolved fluorescence of the flavin cofactor in combination with circular dichroism (both in the flavin fingerprint and far-UV regions) and steady-state fluorescence and absorption spectroscopy. The spectroscopic data show two conformational states of...

Background To explore the antioxidant and hepatoprotective effect of ethanolic Mangifera indica (EMI) and methanolic Mangifera indica (MMI) leaf extracts in mercuric chloride (HgCl2) induced toxicity in Swiss albino mice. Materials and methods Toxicity in mice was induced with HgCl2 (5.0 mg/kg, i.p.), followed by oral intervention with EMI and MMI extracts (25 mg and 50 mg/kg. body wt.) for 3...

The activity of NADH-cytochrome b5 reductase (NADH-methemoglobin reductase) is generally reduced in red cells of patients with recessive hereditary methemoglobinemia. To determine whether this lower activity is due to reduced concentration of an enzyme with normal catalytic properties or to reduced activity of an enzyme present at normal concentration, we measured erythrocyte reductase concentr...

The condition known as male pattern hair loss and benign prostatic hyperplasia are both treated with a family of drugs 5 alpha reductase (5α-reductase) inhibitors. This study shows that the flower Hibiscus rosa sinensis has 5α-reductase inhibitory action is helpful in treatment androgenic diseases. Phytochemical screening was done using petroleum ether, methanol, water extracts to identify phyt...

Demolybdo nitrate reductase (also called cyt c reductase) of Chlorella vulgaris has been converted to active nitrate reductase by insertion of Mo from Na2MoO4 in vitro. A procedure is described which consistently gives about 0.3 unit of nitrate reductase from about 6 units of cyt c reductase, a yield of 30% of the maximum expected, if we calculate on a basis of a ratio of 6 to 1 for the cyt c r...