The largest subunit of RNA polymerase II (RNAPII) C-terminal heptarepeat domain (CTD) is subject to phosphorylation during initiation and elongation of transcription by RNA polymerase II. Here we study the molecular mechanisms leading to phosphorylation of Ser-7 in the human enzyme. Ser-7 becomes phosphorylated before initiation of transcription at promoter regions. We identify cyclin-dependent...

The Med2, Med3 and Med15 proteins form a heterotrimeric subdomain in the budding yeast Mediator complex. This Med15 module is an important target for many gene specific transcription activators. A previous proteome wide screen in yeast identified Med3 as a protein with priogenic potential. In the present work, we have extended this observation and demonstrate that both Med3 and Med15 form amylo...

This paper descripbes an open, flexible infrastructure to support virtual organisations using mediator agents. When applied in businessto-business electronic commerce, the mediators allows partners to exchange rich business information, and act in an agile and coordinated manner. As an example, we have created a demonstration application in the domain of telecommunications service provision, in...

An open distributed environment can be perceived as a service market where services are freely ooered and requested. Any infrastructure which seeks to provide appropriate mechanisms for such environments has to include some mediator functionality to bring together matching service requests and service ooers. The matching algorithm that the mediator must perform commonly builds upon an IDL{based...

The varicella-zoster virus major transactivator, IE62, contains a potent N-terminal acidic transcriptional activation domain (TAD). Our experiments revealed that the minimal IE62 TAD encompasses amino acids (aa) 19 to 67. We showed that the minimal TAD interacts with the human Mediator complex. Site-specific mutations revealed residues throughout the minimal TAD that are important for both acti...

As part of the MKBEEM project, we present an ontology based mediation framework for electronic commerce applications. The framework is based on a mediator/wrapper approach that supports an integrated view over multiple heterogeneous sources. The MKBEEM mediation system allows to fill the gap between customers queries (possibly expressed in a natural language) and diverse specific providers offe...

We investigated whether the multisubunit holoenzyme complex of RNA polymerase II (Pol II) and mediator is universally required for transcription in budding yeast. DeltaCTD Pol II lacking the carboxy-terminal domain of the large subunit cannot assemble with mediator but can still transcribe the CUP1 gene. CUP1 transcripts made by DeltaCTD Pol II initiated correctly and some extended past the nor...

Eukaryotic transcriptional activators work by recruiting to DNA the transcriptional machinery, including protein complexes required for chromatin modification, transcription initiation, and elongation. Which of these complexes must be directly recruited to trigger transcription? We test various "non-classical" transcription activators (comprising a component of the transcriptional machinery fus...

Case-based reasoning is a reasoning method that capitalizes on previous experience. In case-based reosoning, a new problem is solved in a way that is anologous to a previous similar problem. Case-bosed reasoning can improve problem-solving behavior In several ways: by providing reasoning shortcuts, by worning of the potential for error, and by suggesting a focus for reasoning. The MEDIATOR was ...