Plasmodium, Isospora, Toxoplasma, Babesia and Cryptosporidium are parasites which have a significant role in human health. The location in which the protozoon settles in the body, the way that it leaves the host, and the sample which is send from the clinic are the elements of the general methods used in the diagnosis of the ailments caused from protozoan. Classical methods are not adequate for...

Herpetic keratitis which is resulted from herpes simplex virus type I, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. The best method to diagnose it is molecular method which has high sensitivity and exclusivity. The current study was done on 25 eye samples collected from patients referred to the Feyz...

Loop-mediated isothermal amplification (LAMP), an attractive DNA amplification method, was developed as a valuable tool for the rapid detection of Toxoplasma gondii. In this study, species-specific LAMP primers were designed by targeting the AF146527 sequence, which was a conserved sequence of 200- to 300-fold repetitive 529 bp fragment of T.gondii. LAMP reaction system was optimized so that it...

SARS-CoV-2 emerged as a new respiratory virus spreading rapidly to all areas of the world. A systematic review with meta-analysis concerning use Loop-Mediated Isothermal Amplification (LAMP) methodology targeting nucleoprotein (N) gene was conducted. The search resulted in 229 articles, which 19 articles were selected compose final review. In general, LAMP showed high specificity detection and ...

background: parasitological methods for the diagnosis of visceral leishmania-sis (vl) require invasive procedures, so serological and molecular approaches have been developed but are not generally applicable in the field. we evaluated a loop mediated isothermal amplification (lamp) assay using blood from vl pa-tients and compared it to nested pcr. methods: forty-seven subjects with clinical fea...

Bovine viral diarrhea virus (BVDV) is a pathogen that infects cattle, and is globally important. It causes substantial financial losses to the livestock industry. In the current study, a one-step reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) assay was set up for rapid and efficient detection of BVDV. For this purpose, four primers were designed to recognize six distinct...

A Loop-Mediated Isothermal Amplification (LAMP) assay was developed for the detection of pine pathogen Dothistroma septosporum (G. Dorog.) M. Morelet. The specificity LAMP tested using a selection needle fungi, including pini Hulbary, and Lecanosticta acicola (Thüm.) Syd.; only D. DNA amplified by test. In terms sensitivity, able to detect as little 1 pg total DNA. This enables extracted from d...

Influenza virus infections represent a worldwide public health and economic problem due to the significant morbidity and mortality caused by seasonal epidemics and pandemics. Sensitive and convenient methodologies for detection of influenza viruses are essential for further disease control. Loop-mediated isothermal amplification (LAMP) is the most commonly used method of nucleic acid isothermal...

In the present study 28 Arcanobacterium pluranimalium strains isolated from various origins could successfully be identified with a newly designed loop-mediated isothermal amplification (LAMP) assay based on gene pla encoding pluranimaliumlysin. No comparable reaction could be observed with control strains representing five species of genus Arcanobacterium and five species of genus Trueperella....