Forty-five strains of Shigella were screened for haemagglutinin production and broad-spectrum haemagglutination reaction. Mannose-sensitive haemagglutinin (MSHA) was found in 22 strains [Shigella flexneri (7), S. dysenteriae (7), S. sonnei (3), and S. boydii (5)]. Eighteen strains harboured mannose-resistant haemagglutinin (MRHA), and 8 strains were observed to be non-haemagglutinating to guine...

P or Pap fimbriae of avian pathogenic Escherichia coli (APEC) have previously been shown to be important in the pathogenesis of colibacillosis. However, no study has been done to ascertain the effects of deletion of the genes responsible for P fimbrial biogenesis on APEC's in vitro and/or in vivo characteristics. In the present study, all 11 genes of the pap gene cluster were deleted from APEC ...

An outbreak of gastrointestinal illness with clinical and epidemiologic features of enterotoxigenic Escherichia coli (ETEC) occurred among patrons of a restaurant during April 1991. Illnesses among several groups of patrons were characterized by diarrhea (100%) and cramps (79%-88%) lasting a median of 3-5 days. Median incubation periods ranged from 50 to 56 h. A nonmotile strain of E. coli (E. ...

Antibiotic treatment of tuberculosis (TB) is complex, lengthy, and can be associated with various adverse effects. As a result, patient compliance often poor, thus further enhancing the risk selecting multi-drug resistant bacteria. Macrophage mannose receptor (MMR)-positive alveolar macrophages (AM) constitute niche in which Mycobacterium replicates survives. Therefore, we encapsulated levoflox...

Introduction and Aim: In this study, the interaction between Acanthamoeba culbertsoni Shigella sonnei was analyzed. addition, effect of monosaccharides on cytotoxicity A. analyzed through with macrophages. particular, activity protease Materials Methods: Association tests were performed for trophozoites Escherichia coli DH5 alpha, proteolytic enzyme all these tests, monosaccharides, e.g., manno...

The natural history of the mannose 6-phosphate receptor was examined by radiolabeling cells in monolayers or in suspension; the receptor was isolated by immuno- or affinity precipitation followed by polyacrylamide gel electrophoresis. The receptor was found to contain asparagine-linked oligosaccharide chains and phosphorylated serine residues. Newly made receptor was sensitive to endo-beta-N-ac...

Thymus-leukemia (TL) antigens are expressed in murine lymphocytes under strict developmental regulation. To elucidate the molecular basis of TL expression, we have identified the molecular species that react with TL antiserum. At least three species can be resolved by metabolic radiolabeling of thymocytes and ASL1 leukemia cells, lysis, immune precipitation, and sodium dodecyl sulfate-polyacryl...

Normal bovine and mouse sera contain a component, termed beta inhibitor, that inhibits the infectivity and hemagglutinating activity of influenza A viruses of the H1 and H3 subtypes. To investigate the nature of the interaction of beta inhibitors with influenza A viruses we isolated a mutant of the virus Mem71H-BelN (H3N1) that could grow in the presence of bovine serum. The mutant virus was re...

Carboxypeptidase Y and invertase from baker’s yeast, Saccharomyces cerevisiae, have two classes of Nlinked high mannose oligosaccharides which may be distinguished on the basis of their susceptibility to hydrolysis by endo-8-N-acetylglucosaminidase H (Endo H). Thus, three of the four oligosaccharides on carboxypeptidase Y and seven of nine of those on invertase are readily released by Endo H wh...

MDW40, a wheat germ agglutinin-resistant (WGAr) mutant of the highly metastatic tumor cell line called MDAY-D2, is restricted to local growth at the subcutaneous site of inoculation. The WGAr tumor cells acquire metastatic ability by fusing spontaneously with a normal host cell followed by chromosome segregation, a process accompanied by reversion of the WGAr phenotype (i.e., WGAs). Since lecti...