This year marks the 20th anniversary of Koichi Tanaka securing Nobel Prize in chemistry, shared with John Fenn and Kurt Wüthrich, for demonstrating applicability laser technology to analyze biological macromolecules. The principle desorption is fundamental many today’s analytical methods such as matrix-assisted desorption/ionization (MALDI). In this article, we provide an overview MALDI highlig...

BACKGROUND Whole-cell matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) has been successfully applied for bacterial identification and typing of many pathogens. The fast and reliable qualities of MALDI-TOF MS make it suitable for clinical diagnostics. MALDI-TOF MS for the identification and cluster analysis of Streptococcus pyogenes, however, has not ...

BACKGROUND Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and the related technique, surface-enhanced laser desorption/ionization (SELDI)-TOF MS, are being applied widely to analyze serum or plasma specimens for potential disease markers. METHODS Reports on the basic principles and applications of MALDI-TOF MS were reviewed and related to informati...

BACKGROUND Emerging technologies for rapid identification of microbes demonstrate a shift from traditional biochemical and molecular testing algorithms toward methods using mass spectrometry (MS) for the semiquantitative analysis of microbial proteins and genetic elements. This study was performed to assess the diagnostic accuracy of 2 such technologies, PCR-electrospray ionization (ESI)/MS and...

The term “proteome” refers to the total of all proteins expressed in an organism. “proteomics” field research that includes not only information on expression levels individual proteins, but also their higher-order structures, intermolecular interactions, and post-translational modifications. core technology, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), is available...

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), which provides high sensitivity and resolution, has been used for the analysis of biopolymers. In past decade, MALDI-TOF MS utilized in clinical fields around world to identify microbial species. However, serotype or strain discrimination same bacterial species is difficult by MS. Different methods such...

̨-Cyano-4-hydroxycinnamic acid (CHCA) is often selected as the matrix-assisted laser desorption/ionization (MALDI) matrix for peptide analysis. However, CHCA is recognized as a hot matrix,1–5 which is seldom used in the analysis of phosphopeptides. This is because extensive fragmentation from intact analytes is frequently observed in MALDI mass spectra when CHCA is used as the matrix.6,7 However...

Within less than a decade matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become a gold standard for microbial identification in clinical microbiology laboratories. Besides identification of microorganisms the typing of single strains as well as the antibiotic and antimycotic resistance testing has come into focus in order to speed up the microbio...

Rare nonfermenting Gram-negative bacilli, such as Chryseobacterium indologenes and Elizabethkingia meningoseptica, have clinical importance in nosocomial infections and cystic fibrosis (CF), and their identification is a challenge to microbiology laboratories. Thus, the objective of this study was to verify the performance of phenotypic and mass spectrometry (matrix-assisted desorption ionizati...

Highlights 1. In October 2014, three DSM microbiology laboratories received MALDI-ToF instruments for identification of bacterial pathogens and will be implementing this technology in Winnipeg and Brandon over the next several months. 2. MALDI-ToF will result in decreased turnaround times for identification of pathogens, greater accuracy and lower costs of identification. 3. Combined with a pro...