A procedure based on affinity chromatography for the extraction of ocytocin is described. Ocytocin antibody, covalently attached to a magnetizable iron suspension, retains its ability to bind ocytocin from blood plasma. The conjugate can be reused several times. Recovery of ocytocin was 60-70%, and the detection limit for the extraction assay was about 1 pg per tube. The specificity of the extr...

Raising antibodies to a hapten (drug or steroid) requires that it be coupled to a carrier protein through a bridge such that the hapten has maximum exposure with minimum changes in its configuration. In raising antisera in six sheep against carbamazepine (CBZ) coupled to bovine albumin, we found that some subpopulations of the antibodies recognized the bridge linking the drug to the carrier pro...

A fully canonical quantization of electromagnetic field is introduced in the presence of an anisotropic polarizable and magnetizable medium with spatialtemporal dispersion. Two tensor fields which couple the electromagnetic field with the medium and have an important role in this quantization method are introduced. The electric and magnetic polarization fields of the medium naturally are conclu...

We describe a fluoroimmunoassay for progesterone in serum or plasma. The assay involves use of an antiserum to progesterone-11-hemisuccinate and a labeled antigen prepared by linking fluoresceinamine to progesterone-3-carboxymethyloxime by use of a mixed-anhydride procedure. Serum or plasma samples are extracted with hexane. After incubation of a portion of the evaporated extract with antiserum...

A canonical relativistic formulation is introduced to quantize electromagnetic field in the presence of a polarizable and magnetizable moving medium. The medium is modeled by a continuum of the second rank antisymmetric tensors in a phenomenological way. The covariant wave equation for the vector potential and the covariant constitutive equation of the medium are obtained as the EulerLagrange e...

Radioimmunoassays for haloperidol are described, involving use of tritium- or 125I-labeled drug or tritium-labeled spiroperidol, and a rabbit antiserum to a drug/bovine serum albumin conjugate. The 125I-labeled drug was prepared by the Chloramine T iodination technique. A fluoroimmunoassay for haloperidol is also described in which the antiserum is coupled to magnetizable solid-phase medium, an...

Magnetorheological (MR) uids represent a class of smart materials whose rheological properties change in response to the application of a magnetic eld. These uids typically consist of small (m) magnetizable particles dispersed in a non-magnetic carrier uid that generally contains additives such as surfactants and anti-wear agents 1]. Due to such additives , there is an outer non-magnetic layer ...

We describe a fully automated continuous-flow radioimmunoassay for methotrexate. [125I]Histamine-labeled methotrexate was used as tracer. Anti-methotrexate serum was coupled to a magnetizable solid-phase and the bound and free fractions were separated with an electromagnetic field. The assay is precise (CV less than 2.5%) and rapid (30 samples per hour), incubation volume is small (about 160 mi...

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