The adsorption of arsenic(V) was investigated using macroporous resin beads containing magnetite crystals. Arsenic(V) was favorably adsorbed at pH 2-9, where the distribution coefficients were larger than 10(3). The maximum capacity was 0.050 mmol/g. Metal cations including Ca(II), Mn(II), Co(II), Ni(II), Cu(II), Zn(II) and La(III) did not give serious interference at 10(-4) M level. Diluted ar...

A novel and industrially applicable strategy for separating and purifying the water-soluble polysaccharides from the fresh Spirulina platensis is introduced in this study. After hot water extraction, using chitosan flocculation treatment, 86.0% of the particles and 85.9% of the pigments in the crude extract were removed. The purity of polysaccharides could be increased to 94.6% and 96.1% by app...

Protein A affinity chromatography is a central part of most commercial monoclonal antibody and Fc-fusion protein purification processes. In the last couple years an increasing number of new Protein A technologies have emerged. One of these new Protein A technologies consists of a novel, alkaline-tolerant, Protein A ligand coupled to a macroporous polymethacrylate base matrix that has been optim...

Flowers of Carthamus tinctorius L. are traditionally used in China to treat cerebrovascular and cardiovascular diseases. Hydroxysafflor yellow A (HSYA), the main constituent of Carthamus tinctorius L. flowers, is known for its multiple biological activities. In the present study, HSYA was isolated from Carthamus tinctorius L. flowers by a macroporous resin adsorption chromatography method coupl...

The efficacy of Prunella extracts in the prevention and treatment of lung cancer has been attributed to different components. In this study, an "active components combination model" hypothesis was proposed to explain the anti-tumor activity of Prunella. The efficacy of Prunella extracts from different regions was compared in vitro and in vivo, and the TNF-α activity in serum of tumor-bearing mi...

We used two methods of reversed-phase liquid chromatography with amperometric detection, one for assay of unconjugated norepinephrine, epinephrine, and dopamine in urine, the other for determination of unconjugated vanillylmandelic acid and homovanillic acid. The catecholamines were extracted from urine by passage through a weak-cation exchange resin, then through alumina. Vanillylmandelic acid...

The Jaff#{233} reaction is commonly used to determine the concentration of creatinine in urine and serum by measuring the color produced when picric acid and sodium hydroxide solutions are combined with urine or protein-free filtrate from serum. As is well known, substances other than creatinine react to give positive error, the relative amount of interference usually being much greater in seru...

self etching primers, due to ease of manipulation, have been extensively used in recent years. these self etching primers containing a relatively weak acid, may not provide an optimal bond strength. the purpose of the present in-vitro investigation was to evaluate the effects of 37% phosphoric acid application prior to applying self etching primers on composite bond strength. in this experiment...

Dried unripe raspberries (Rubus chingii), a kind of Chinese herbal medicine, have been used for many years in China. They were prepared as unpurified raspberry extract (URE) by ethanol extraction, and purified (PRE) macroporous resin. Synergistic effects between the URE (or PRE) ampicillin, erythromycin, tetracycline or streptomycin against Escherichia coli, Staphylococcus aureus Salmonella ent...

OBJECTIVES Pistacia lentiscus has traditionally been used in the treatment of many diseases. Its resin was investigated for its mineral contents, anti-inflammatory and antioxidant activities in rats. MATERIAL AND METHODS Inhibition of carrageenan induced edema was used to evaluate anti-inflammatory activity. Fe2+ chelating ability, 1,1-diphenyl-2-picryl hydrazyl radical (DPPH) and nitric oxid...