Lysozyme is a major secretory product of human and rodent macrophages and a useful marker for myelomonocytic cells. Based on the known human lysozyme amino acid sequence, oligonucleotides were synthesized and used as probes to screen a phorbol 12-myristate 13-acetate-treated U937 cDNA library. A full-length human lysozyme cDNA clone, pHL-2, was obtained and characterized. Sequence analysis show...

The antimicrobial peptide lysozyme is an important factor of innate immunity and exerts high potential of antibacterial activity. In the present study we evaluated the lysozyme expression in serum of multiple injured patients and subsequently analyzed their possible sources and signaling pathways. Expression of lysozyme was examined in blood samples of multiple trauma patients from the day of t...

Inclusion of protein lysozyme molecules in the lipidic mono-olein cubic phase induces a transition from a Pn3m structure to an Im3m one. The small-angle x-ray scattering method with high-intensity synchrotron radiation enabled us to follow closely the transition depending on the conditions of lysozyme solutions. We show that concentrated lysozyme solutions induced the appearance of the Im3m str...

The aim of this study was to elucidate the influences of parity, age and mineral and trace elementmixture-supplementation on the course of lysozyme activity in mare’s milk during early lactation period. The interrelationship between equine milk lysozyme activity and calcium content was also tested. Lysozyme activity was measured by the modified turbidimetric method using Micrococcus lysodeiktik...

A novel protein imprinted polymer for selective recognition of lysozyme was obtained. Acryloyl-beta-cyclodextrin, which offered hydrophilic exterior and hydrophobic cavity that were allowed to self-assemble with the template protein through hydrogen interaction and hydrophobic interaction, was synthesized and used as the functional monomer. Polymerization was carried out in the presence of acry...

Human lysozyme variants form amyloid fibrils in individuals suffering from a familial non-neuropathic systemic amyloidosis. In vitro, wild-type human and hen lysozyme, and the amyloidogenic mutants can be induced to form amyloid fibrils when incubated under appropriate conditions. In this study, fibrils of wild-type human lysozyme formed at low pH have been analyzed by a combination of limited ...

The surfactant precipitation and recovery method (SPR method) consist of a step, that is protein-surfactant complex by the addition aqueous solution to protein solution, dissolution precipitate in polar organic solvent trace amount salt solution. When denaturant was not included, ratio lysozyme increased as AOT/lysozyme molar increased, after reaching 100%, decreased due redissolution precipita...

Conformational investigations have been carried out on derivatives of lysozyme in which tyrosines 20 and 23 were nitrated (NTa-lysozyme), or in which the nitrotyrosine residues had been reduced to aminotyrosine (ATn-lysozyme). Also, a derivative modified at the 6 tryptophan residues by reaction with 2-nitrophenyl sulfenyl chloride (NPSe-lysozyme) was studied. In optical rotatory dispersion meas...

INTRODUCTION We compared the salivary immunoglobulin A (IgA) and lysozyme concentration and secretion rates among mild and severe psoriasis patients and controls in Singapore. MATERIALS AND METHODS Fifty-one psoriasis patients and 24 controls participated in the study. None of the patients were on immunosuppressive therapy. The Psoriasis Area and Severity Index (PASI) was used to assess the s...

This study demonstrates the induction of lysozyme mRNA expression in situ in tissue macrophages (M phi) of mice following in vivo stimulation. The resting resident tissue M phi of most tissues do not contain enough lysozyme mRNA to be detected by in situ hybridization using 35S-labeled RNA probes. Following Bacille Calmette Guerin or Plasmodium yoelli infection, however, M phi recruited to live...